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ISOLATION AND ANALYSIS OF URATE TRANSPORTER OF HUMAN PROXIMAL TUBULE

ISOLATION AND ANALYSIS OF URATE TRANSPORTER OF HUMAN PROXIMAL TUBULE
人体近端肾小管尿酸盐转运蛋白的分离与分析
批准号:
10671010
负责人:
ICHIDA Kimiyoshi
金额:
$1.22万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999

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中文摘要
翻译
方法:一个hOAT 1表达鼠标近似管细胞的第二段线已在本研究中得到证实和使用。通过测量YI-D114 Y-D1C-urate的细胞含量,通过hOAT 1的urate传输特征。结果:YI-D1C-urate传输的Km值和Vmax值通过hOAT 1的值为943±84 μM和1286±162 pmo 1/mg蛋白质/min,尊重结论:作为第一台克隆的人类运输机,我们通过hOAT 1确定了实时运输的特征。
英文摘要
Methods : A hOAT1 expressing mouse proximal tubule cell line of second segment was established and used in this study. By measuring the cellular uptaked of ィイD114ィエD1C-urate, characteristics of urate transport via hOAT1 were clarified.Results : The Km value and the Vmax value of ィイD114ィエD1C-urate transport via hOAT1 were 943±84 μM and 1286±162 pmo1/mg protein/min, respectively.Conclusions : As the first cloned human urate transporter, we clarified the characteristics of urate transport via hOAT1.
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Identification and analysis of genes influencing serum uric acid levels
Elucidation of mechanism for exercise-induced acute renal failure in urate transporter deficient mice
Genetic analysis for mechanism of underexcretion type hyperuricemia
  • 批准号:
    14571041
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $1.86万
  • 财政年份:
    2002
  • 负责人:
    ICHIDA Kimiyoshi
  • 依托单位:
海外基金