Homocysteine metabolism and oxygen stress in chronic renal failure

慢性肾衰竭中的同型半胱氨酸代谢和氧应激

• 批准号: 10671013
• 负责人: TAKAMITSU Yoshihiro
• 金额: $ 0.32万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671013/
• 关键词: progression of renal failure; oxygen stress; homocysteine; cysteine; nitric oxide; urine protein; macrophage; マクロファージ; 還元型GSH(グルタチオン); カタラーゼ; 尿細管細胞

We have already demonstrated that homocysteine (Hcy) and NO metabolites (NO3) are increased, as the renal function is decreased. Hcy and cysteine (Cys), those are amino acids with SH-base, as well as NO might accelerate the generation of O2-・, OH・ and peroxynitrite. As the rise in oxygen radical is associated with the deterioration of renal function, we determined the relation between sulfur amino acids, as well as NO metabolites and the rate of deterioration of renal function in patients with chronic renal failure. Serum creatinine level was over 2.5 mg/dl in all patients, who have been followed up for at lease 9 months. The rate of deterioration of renal function was expressed as the timed change of reciprocal of creatinine (SL-ICR). SL-ICR was not correlated with pHcy, but was invertedly correlated with pCys and pCys/pHcy. And it was significantly correlated with the daily excretion of urine protein and pNO3. From these results, the deterioration of renal function might be related with sulfur amino acids metabolism, especially cysteine synthesis. On the other hand, renal function decreased rapidly as NO synthesis increased. It might be suggested that NO synthesis from macrophage in renal tissue might injure the renal function.

我们已经证明，随着肾功能的降低，同型半胱氨酸（HCY）和没有代谢物（NO3）的增加。 Hcy和半胱氨酸（Cys），这些是具有SH碱基的氨基酸，也可能会加速O2- ・，OH ・和过氧亚硝酸盐的产生。由于氧自由基的升高与肾功能的恶化有关，因此我们确定了硫氨基酸以及无代谢产物的关系以及慢性肾衰竭患者的肾功能的确定率。所有患者的血清肌酐水平均超过2.5 mg/dL，他们在9个月内接受了租赁的遵循。肾功能的确定速率表示为肌酐倒数（SL-ICR）的定时变化。 SL-ICR与PHCY无关，而与PCYS和PCY/PHCY倒数相关。它与尿液蛋白和PNO3的每日排泄显着相关。从这些结果中，肾功能的定义可能与硫氨基酸的代谢，尤其是半胱氨酸合成有关。另一方面，随着无合成的增加，肾功能迅速降低。可能建议，肾脏组织中没有巨噬细胞的合成可能会损害肾功能。