Expression of UCP-2 and UCP-3 genes in obese type 2 diabetes mellitus and its modification by a cytokine inducer

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Expression of UCP-2 and UCP-3 genes in obese type 2 diabetes mellitus and its modification by a cytokine inducerKK-Ay mouse is a model of obese type 2 diabetes mellitus. Glucose intolerance of KK-Ay mice was significantly improved by CFA treatment and the improvement was medicated by IL-1. CFA-treated KK-Ay mice lost body weight as compared to saline-treated control mice even though food intake was not changed, suggesting increase of energy expenditure in CFA-treated KK-Ay mice. To observe the increase of energy expenditure, body temperature, oxygen consumption and heart rate were measured in CFA-treated and control mice. However, there was no significant different in these measurements between both groups. mRNA expression of UCP-2 and UCP-3 was examined in C57/BL mice and KK-Ay mice. There was a tendency that expression of UCP-2 mRNA in the spleen, white adipose tissue and muscle were lower in KK-Ay mice than those in C57/BL mice and these were increased by CFA treatment. Further experiments were in progress to confirm these results. Finally, to screen obese patients with type 2 diabetes mellitus for abnormal energy metabolism, we measured temperature of tympanitic membrane before and after meals. However, we could not find obviously abnormal patients in the temperature change, because of unstable changes with wide range.