Expression of UCP-2 and UCP-3 genes in obese type 2 diabetes mellitus and its modification by a cytokine inducer

UCP-2和UCP-3基因在肥胖2型糖尿病中的表达及其细胞因子诱导剂的修饰

• 批准号: 10671053
• 负责人: SATOH Jo
• 金额: $ 2.11万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671053/
• 关键词: NIDDM; type 2 diabetes; KK-Ay mouse; CFA; GTT; UCP-2; IL-1

Expression of UCP-2 and UCP-3 genes in obese type 2 diabetes mellitus and its modification by a cytokine inducerKK-Ay mouse is a model of obese type 2 diabetes mellitus. Glucose intolerance of KK-Ay mice was significantly improved by CFA treatment and the improvement was medicated by IL-1. CFA-treated KK-Ay mice lost body weight as compared to saline-treated control mice even though food intake was not changed, suggesting increase of energy expenditure in CFA-treated KK-Ay mice. To observe the increase of energy expenditure, body temperature, oxygen consumption and heart rate were measured in CFA-treated and control mice. However, there was no significant different in these measurements between both groups. mRNA expression of UCP-2 and UCP-3 was examined in C57/BL mice and KK-Ay mice. There was a tendency that expression of UCP-2 mRNA in the spleen, white adipose tissue and muscle were lower in KK-Ay mice than those in C57/BL mice and these were increased by CFA treatment. Further experiments were in progress to confirm these results. Finally, to screen obese patients with type 2 diabetes mellitus for abnormal energy metabolism, we measured temperature of tympanitic membrane before and after meals. However, we could not find obviously abnormal patients in the temperature change, because of unstable changes with wide range.

肥胖2型糖尿病中UCP-2和UCP-3基因的表达及细胞因子诱导的修饰KK-Ay小鼠是一种肥胖型2型糖尿病模型。CFA可明显改善KK-Ay小鼠的糖耐量，这种改善作用可被IL-1拮抗。尽管食物摄入量没有改变，但与生理盐水对照组相比，CFA处理的KK-Ay小鼠体重减轻，这表明CFA处理的KK-Ay小鼠的能量消耗增加。为了观察能量消耗的增加，测定了CFA治疗组和对照组小鼠的体温、耗氧量和心率。然而，在这些测量中，两组之间没有显著差异。检测C57/BL小鼠和KK-Ay小鼠UCP-2和UCP-3的mRNA表达。KK-Ay小鼠的脾、白色脂肪组织和肌肉中UCP-2mRNA的表达有低于C57/BL小鼠的趋势，经CFA治疗后，这些表达均有增加的趋势。进一步的实验正在进行中，以证实这些结果。最后，为了筛查肥胖2型糖尿病患者的能量代谢异常，我们测量了餐前和餐后鼓膜温度。但由于温度变化范围大，变化不稳定，我们在体温变化中没有发现明显的异常。