Study on a new gene therapy -Using liposome attached hemagglutinin and included anti-oncogene

1)本研究利用本实验室建立的8株食管癌细胞系和7株食管癌裸鼠移植瘤共15个样本细胞，研究了腺病毒介导的野生型p53基因转染人食管癌细胞的生长情况。此外，我还尝试用15个样本细胞的面板研究脂质体附着的血凝素和包含抑癌基因的人食管癌细胞的生长。2）结果，在这些细胞系中，结果略有差异，但所有细胞系都表现出抑制趋势。在通过腺病毒使用的情况下认识到这种趋势，并且在通过脂质体使用的情况下认识到更大的抑制趋势。3）为了进一步研究，我们从几例食管癌患者的食管癌组织中收集了可培养的细胞系和裸鼠移植瘤。患者的血清也被收集和储存。

1) I have attempted to study the cell growth by adenovirus-mediated transfer of the wild-type p53 in human esophageal carcinoma cells using a pannel of 15 sample cells consisting of 8 cell lines and 7 nude mouse-transplantable esophageal caricinomas which were established for culture in vitro and trasplant in nude mice in our laboratory, respectively. Moreover I have attempted to study the cell growth by liposome attached hemagglutinin and included anti-oncogene in human esophageal carcinoma cells using a pannel of 15 sample cells.2) As a result, minor difference occurred in the results during the these cell lines, but all cell lines showed suppressive tendency. This tendency was recognized in case of using by adenovirus, and more suppressive tendency was recognized in case of using by liposome. However, I am not able to obtaine stable liposome.3) To further studies, esophageal carcinoma tissues haved been collected from several patients and established as culturable cell lines and transplantable carcinomas in nude mice. Sera of the patients have also been collected and stored.