Study on a new gene therapy -Using liposome attached hemagglutinin and included anti-oncogene

新型基因疗法的研究——脂质体附着血凝素并包含抗癌基因

• 批准号: 10671166
• 负责人: SAITO Mitsukazu
• 金额: $ 1.86万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671166/
• 关键词: gastrointestinal cancer cells; hemagglutinin; liposome; gene therapy; anti-oncogene

1) I have attempted to study the cell growth by adenovirus-mediated transfer of the wild-type p53 in human esophageal carcinoma cells using a pannel of 15 sample cells consisting of 8 cell lines and 7 nude mouse-transplantable esophageal caricinomas which were established for culture in vitro and trasplant in nude mice in our laboratory, respectively. Moreover I have attempted to study the cell growth by liposome attached hemagglutinin and included anti-oncogene in human esophageal carcinoma cells using a pannel of 15 sample cells.2) As a result, minor difference occurred in the results during the these cell lines, but all cell lines showed suppressive tendency. This tendency was recognized in case of using by adenovirus, and more suppressive tendency was recognized in case of using by liposome. However, I am not able to obtaine stable liposome.3) To further studies, esophageal carcinoma tissues haved been collected from several patients and established as culturable cell lines and transplantable carcinomas in nude mice. Sera of the patients have also been collected and stored.

1)我尝试通过腺病毒介导的野生型p53在人食管癌细胞中的转移，使用我们实验室分别建立的用于体外培养和裸鼠移植的8个细胞系和7个裸鼠可移植食管癌15个样本细胞。此外，我还尝试通过脂质体附着血凝素和包含抗癌基因在人食管癌细胞中的生长，使用了15个样本细胞的面板。2)结果表明，各细胞系间的结果差异不大，但均表现出抑制的趋势。在腺病毒使用的情况下，发现了这种趋势，而在脂质体使用的情况下，发现了更大的抑制趋势。然而，我无法获得稳定的脂质体。3)为了进一步研究，我们收集了几例食管癌患者的组织，建立了可培养细胞系和裸鼠可移植癌。患者的血清也已收集和保存。