Study on a new gene therapy -Using liposome attached hemagglutinin and included anti-oncogene

1)本实验室分别建立了人食道癌的体外培养和裸鼠移植瘤模型，共15个样本细胞，包括8个细胞系和7个裸鼠可移植瘤细胞，通过腺病毒介导的野生型P53基因的转移，研究了野生型P53基因对人食管癌细胞生长的影响。此外，我还尝试用15个样本细胞来研究脂质体结合血凝素和含有抑癌基因的细胞在人食道癌细胞中的生长。2)结果表明，不同细胞系之间的结果差异不大，但所有细胞系都表现出抑制趋势。以腺病毒为载体时，可识别出这一趋势，而以脂质体为载体时，其抑制倾向更强。3)为了进一步研究，已从多位患者采集食道癌组织，并建立了可培养细胞系和裸鼠移植癌模型。还收集和储存了患者的血清。

1) I have attempted to study the cell growth by adenovirus-mediated transfer of the wild-type p53 in human esophageal carcinoma cells using a pannel of 15 sample cells consisting of 8 cell lines and 7 nude mouse-transplantable esophageal caricinomas which were established for culture in vitro and trasplant in nude mice in our laboratory, respectively. Moreover I have attempted to study the cell growth by liposome attached hemagglutinin and included anti-oncogene in human esophageal carcinoma cells using a pannel of 15 sample cells.2) As a result, minor difference occurred in the results during the these cell lines, but all cell lines showed suppressive tendency. This tendency was recognized in case of using by adenovirus, and more suppressive tendency was recognized in case of using by liposome. However, I am not able to obtaine stable liposome.3) To further studies, esophageal carcinoma tissues haved been collected from several patients and established as culturable cell lines and transplantable carcinomas in nude mice. Sera of the patients have also been collected and stored.