Study on the evaluation of malignancy of esophageal carcinoma with a special referance to the detection of carcinogenic virus gene and intracelluar organella

1)本实验室建立了8株人食管癌细胞系和7株人食管癌裸鼠移植瘤细胞系，共15个样本细胞，分别检测了食管癌细胞中几种病毒基因的存在和表达。作为用于杂交的病毒DNA探针，使用从日本癌症研究资源库获得的人乳头瘤病毒（1a、2a、4、6、11、16和18型）、EBV、SV 40和HBV。2）用同样的细胞培养板，研究了食管癌细胞对高温的温度敏感性与线粒体数目、琥珀酸脱氢酶（SD）活性和荧光强度的关系。结果表明，在这些线粒体标记物中，FI与高温敏感性的相关性最好。3）为了进一步研究，我们从几个病人身上收集了食管癌组织，并建立了可培养的细胞系和裸鼠移植瘤。患者的血清也被收集和储存。

1) We have attempted to detect the presence and expression of several virus genes in human esophageal carcinoma cells using a pannel of 15 sample cells consisting of 8 cell lines and 7 nude mouse-transplantable esophageal caricinomas which were established for culture in vitro and trasplant in nude mice in our labratory, respectively. As for virus DNA probes for the hybridazation, human papilloma viruses (types 1a, 2a, 4,6,11,16 and 18), EBV,SV40 and HBV,obtained from Japanese Cancer Research REsources Bank, were used. However, the presence and expression of these virus genes were failed to detect in the used esophageal carcinoma cells.2) Using the same cell pannel, the relation between temperature sensitivity on the hyperthermia and number ofmitochondoria, succinate dehydrogenase (SD) activity or fluorescence intensity (FI) after staining with Rhodamine 123 has been examined. As a result, FI shows the best correlation with sensitivity on hyperthermia among these mitochondorial markers3) To further studies, esophageal carcinoma tissues haved been collected from several patients and established as culturable cell lines and transplantable.carcinomas in nude mice. Sera of the patients have also been collected and stored.