Development of the research method for bacterial translocation with indigenous bacteria

在这个研究项目中，我们培育了本土细菌，并命名为JNW14。从Wistar大鼠粪便中分离的JNW14用耐杆菌肽、新霉素和链霉素标记物进行标记。利用本地细菌（JNW14）进行细菌易位（BT）的研究是有益的。利用JNW14进行的动物实验确定了梗阻性黄疸的BT系统。梗阻性黄疸下肝大切除可促进BT向全身脏器转移，肺培养阳性组肠系膜淋巴结活JNW14数量显著高于阴性组，提示BT主要途径为淋巴-胸系统。梗阻性黄疸和肝切除术时网状内皮系统功能受损，其主要原因是肝脏内细菌摄取减少。梗阻性黄疸大鼠回肠形态结构异常，肠通透性增高。在梗阻性黄疸状态下，胆酸和去氧胆酸均未降低BT，但全胆可降低肠道通透性，抑制BT。

In this research project, we developed the indigenous bacteria and named JNW14. JNW14 originally isolated from Wistar rat feces was labeled with bacitracin-, neomycin-and streptomycin-resistant markers.The method using indigenous bacteria (JNW14) was useful to study for bacterial translocation (BT).The animal study using JNW14 identified the BT system in obstructive jaundice.Major liver resection under obstructive jaundice promoted BT to systemic organs and the number of viable JNW14 in the mesenteric lymph nodes in the lung culture positive group was significantly higher than that of negative group, suggesting the major route of BT is lymphatic-thoracic system.The reticuloendothelial system function was impaired in obstructive jaundice and hepatectomy, and it was mainly due to the decrease of the uptake of bacteria in the liver.Morphology of the ileum showed structural abnormalities and increased intestinal permiability in obstructive jaundiced rat.The administration of cholic acid and deoxycholic acid were not decreased BT, but the whole bile decresed intestinal permiability and inhibited BT under obstrustive jaundice state.