Is skeltal muscle cell inplantation able to prevent the irreversible dysfunction of ischemic heart?

点击翻译按钮获取中文摘要

The purpose of this study is to find some interactions during the skeltal muscle cell inplantation into hearts.Through the experiences of our adult rat heart cell cultures, we found that usual culture method for heart cells wasn't suitable for our purpose because heart cells on a dish didn't contract synchronously with cells surrounding it. So we have switched culture method to tri-dimentional culture from flat one.Under the tri-dimentional culture condition, cells maintained their life as long as two months and a lot of gap junctions were observed under fluorescent microscopy using conexin antibody. Then we named the cultured cluster as a functioning cluster because it kept an ability to contract synchronously quite well.By this culture method, we have examined the appropriate condition for the co-culture of primary rat heart cells with skeltal muscle myoblasts (L6 cells), then finaly found the best ratio and numbers for the mixed culture. And a result, morphological changes resembled to heart cells were observed in DAPI labeled L6 cells.