The interaction of IGF and IGF-binding proteins in repairing of cartilage tissue

IGF 和 IGF 结合蛋白在软骨组织修复中的相互作用

• 批准号: 10671366
• 负责人: ENOMOTO Hiroshi
• 金额: $ 1.98万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671366/
• 关键词: chondrocytes; Cartilage tissue; Insulin like growth factor; IGF binding protein; 再生

Insulin-like growth factor-I (IGF-I) is an important anabolic factor for cartilage tissue and its action is in part regulated by IGF-binding proteins (IGFBPs). The object of this study was to investigate the effects of IGFBPs on IGF-I action and on binding of IGF-I to cells using a reproducible immortalized human chondrocyte culture model. Treatment of the C-28/I2 cells with IGF-I or des (1-3 ) IGF-I in serum-free medium stimulated cell proliferation in a dose-dependent manner. However, the effect of des (1-3) IGF-I was more potent, thereby suggesting that endogenously produced IGFBPs inhibited IGF action. The stimulatory effect of IGF-I was inhibited significantly by addition of IGFBP-3. Binding of [125I] IGF-I to the cells was displaced by IGFBP-3. Our findings indicate that the balance between IGFBPs, IGF receptor binding and its action on chondrocyte proliferation may modulate cartilage tissue repair.

胰岛素样生长因子-I (IGF-I) 是软骨组织的重要合成代谢因子，其作用部分受 IGF 结合蛋白 (IGFBP) 调节。本研究的目的是使用可重复的永生化人类软骨细胞培养模型来研究 IGFBP 对 IGF-I 作用以及 IGF-I 与细胞结合的影响。在无血清培养基中用IGF-I或des(1-3)IGF-I处理C-28/I2细胞以剂量依赖性方式刺激细胞增殖。然而，des (1-3) IGF-I 的作用更有效，从而表明内源产生的 IGFBP 抑制 IGF 作用。添加IGFBP-3可显着抑制IGF-I的刺激作用。 [125I] IGF-I 与细胞的结合被 IGFBP-3 取代。我们的研究结果表明，IGFBP、IGF 受体结合及其对软骨细胞增殖的作用之间的平衡可能会调节软骨组织修复。

项目成果

Yonekura A,: "Transforming growth factor-β stimualates articular chondrocytes cell growth through P44/42 MAP kinasa (ERK) activation"Endocrine J. 46. 545-553 (1999)

Yonekura A，：“转化生长因子-β 通过 P44/42 MAP 激酶 (ERK) 激活刺激关节软骨细胞生长” Endocrine J. 46. 545-553 (1999)

Matsumoto T,: "Increased levels of IGF-I and IGFBP-3 in synoyial fluids of patients with rheumatoid arthritis." Endocrine Journal. 45. S141-S144 (1998)

Matsumoto T：“类风湿性关节炎患者滑液中 IGF-I 和 IGFBP-3 的水平升高。”

Matsumoto T: "Increased levels of IGF-I and IGFBP-3 in synovial fluids of patients with rheumatoid arthritis"Endocrine Journal. 5(suppl). 141-144 (1998)

Matsumoto T：“类风湿性关节炎患者滑液中 IGF-I 和 IGFBP-3 水平升高”内分泌杂志。

Chamoun D: "Transcriptional and posttranscriptional regulation of intraovarian insulin-like growth factor- binding proteins by interleukin-1 bet a (IL-1 beta): evidence for IL- 1beta as antiatretic principal"Endocrinology. 140. 3488-3495 (1999)

Chamoun D：“白细胞介素 1 beta (IL-1 beta) 对卵巢内胰岛素样生长因子结合蛋白的转录和转录后调节：IL-1beta 作为抗闭锁主要药物的证据”内分泌学。

Okano K: "Effect of 22-Oxa-1,25 Dihydroxy Vitamin D_3 on Human Thyroid Cancer Cell growth"Endocrine J. 46. 243-252 (1999)

Okano K：“22-Oxa-1,25 二羟基维生素 D_3 对人甲状腺癌细胞生长的影响”Endocrine J. 46. 243-252 (1999)