The effects of anesthetics on skin incision induced WDR neuronal activity in cat

麻醉剂对猫皮肤切口诱导的 WDR 神经元活动的影响

• 批准号: 10671442
• 负责人: NAGASAKA Hiroshi
• 金额: $ 0.64万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671442/
• 关键词: Spinal cord; Ketamine; WDRneuron

Adult cats of both sexes were used in all experiments. The experimental protocol was approved by our Institutional Animal Care and Use Committee and the ethical guidelines for the treatment of animals of the International Association for the Study of Pain were followed. All surgical procedures were performed under halothane - nitrous oxide -oxygen anesthesia. We investigated the effects of ketamine on the activity of WDR neurons in the spinal dorsal horn that was evoked by skin incision in cats. In addition, we studied the effects of pre- and post-administration of ketamine on the maintained phase of the skin incision response. Each dose was a subanesthetic or anesthetic. (3.0 mg kgィイD1-1ィエD1, 10 mg kgィイD1-1ィエD1). Intravenous administered ketamine produced a dose dependent depression of evoked activity that was not significantly greater when drug was administered before skin incision. However, we can't observe a benefit of a clear preemptive effect of ketamine in cat.

在所有实验中使用了两种性别的成年猫。实验方案由我们的机构动物护理和使用委员会批准，并遵循国际疼痛研究协会的动物治疗伦理指南。所有手术均在氟烷-氧化亚氮-氧麻醉下进行。本实验观察了氯胺酮对猫皮肤切口诱发的脊髓背角WDR神经元活动的影响。此外，我们研究了氯胺酮给药前后对皮肤切口反应维持期的影响。每次给药均为亚麻醉剂或麻醉剂。(3.0 10 mg kg kg kg D1 -1 kg D1）。静脉注射氯胺酮可产生剂量依赖性的诱发活动抑制，但在皮肤切开前给药时抑制程度并不显著更大。然而，我们无法观察到氯胺酮在猫中具有明确的先发制人作用的益处。

项目成果

Nagasaka H et al: "Effect of ketamine on formalin-induced activity in the spinal dosal horn of spinal cord-transected cat"Acta Anaesth. Scand. (in press). (2000)

Nagasaka H 等人：“氯胺酮对脊髓横断猫脊髓背角福尔马林诱导活动的影响”Acta Anaesth。

DL DeHaven-Hudkin: "ADL2-1294,an opioid antihyperlgesic agent with peripheral selectivity" J Pharmacol Exp ther. (1999)

DL DeHaven-Hudkin：“ADL2-1294，一种具有外周选择性的阿片类抗过敏药”J Pharmacol Exp ther。

H.Nagasaka: "Effects of ketamine on formalin induced activity in the spinal dorsal horn of spinal cord-transeited cats."Ata Anaesth Scand. (in press). (2000)

H.Nagasaka：“氯胺酮对福尔马林诱导的脊髓转运猫脊髓背角活动的影响。”Ata Anaesth Scand。

初雁育介、長坂浩他: "AVMを合併した妊婦の麻酔経験"麻酔. 49. 33-36 (2000)

Ikusuke Hatsugan、Hiroshi Nagasaka 等：“AVM 孕妇的麻醉经验” 麻醉 49. 33-36 (2000)。

初雁育介、長坂 浩その他: "脳動静脈奇形(AVM)を合併した妊婦の麻酔経験"麻酔. 49. 33-36 (2000)

Ikusuke Hatsugan、Hiroshi Nagasaka等：“脑动静脉畸形（AVM）孕妇的麻醉经验”麻醉49. 33-36（2000）。