Autophagy-inducing activity of anesthetics against various malignant cells

麻醉剂对多种恶性细胞的自噬诱导活性

• 批准号: 21591987
• 负责人: NAGASAKA Hiroshi
• 金额: $ 2.33万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591987/
• 关键词: 麻酔; 蘇生学; オートファジー; アポトーシス; ネクローシス; 癌細胞; dexmedetomidine; clonidine; adrenaline; がん細胞; シダゾラム; ジブカイン; リドカイン; アドレナリン

Anesthetics have been administered to malignant tumors and surrounding tissues during the surgery, their effects on oral tissues is not well understood. In the present studies, the cytotoxicity of 1) local anesthetics, such as dibucaine 2) intravenous anesthetics, such as midazolam and 3) adrenergic agonists, such as dexmedetomidine against oral tumor and normal cells was compared among each group. Tumor-specificity index was determined by the ratio of mean 50% cytotoxic concentration against normal cells to that for tumor cells. Apoptosis induction was monitored by internucleosomal DNA fragmentation and caspase-3,-8, and-9 activation. Fine cell structure was observed under transmission electron microscopy. Dibucaine, midazolam and dexmedetomodine did not induce apoptosis in oral squamous cell carcinoma(OSCC) cells although they induced apoptosis in HL-60(human promyelocytic leukemia). Autophagy inhibitors did not reduce the cytotoxicity induced by these drugs. The necrosis may be involved in the induction of antitumor activity by local anesthetics, intravenous anesthetics and adrenergic agonists in OSCC cells. However, further study is needed for another tumor cell lines

在手术过程中，麻醉剂已被用于恶性肿瘤及其周围组织，其对口腔组织的影响尚不清楚。在本研究中，比较了各组中1）局部麻醉剂（如地布卡因）2）静脉麻醉剂（如咪达唑仑）和3）肾上腺素能激动剂（如右美托咪定）对口腔肿瘤和正常细胞的细胞毒性。肿瘤特异性指数由对正常细胞的平均50%细胞毒性浓度与对肿瘤细胞的平均50%细胞毒性浓度的比值确定。通过核小体间DNA片段化和caspase-3、caspase-8和caspase-9激活来监测细胞凋亡诱导。透射电子显微镜下观察到细小的细胞结构。地布卡因、咪达唑仑和右美托莫定虽然诱导HL-60（人早幼粒细胞白血病）细胞凋亡，但不诱导口腔鳞状细胞癌（OSCC）细胞凋亡。自噬抑制剂不能降低这些药物诱导的细胞毒性。这种坏死可能与局部麻醉药、静脉麻醉药和肾上腺素能受体激动剂诱导OSCC细胞抗肿瘤活性有关。但对其他肿瘤细胞系的研究尚需进一步深入

项目成果

Tumor-specific cytotoxicity and type of cell death induced by benzaldehyde.

苯甲醛诱导的肿瘤特异性细胞毒性和细胞死亡类型。

• 发表时间: 2010
• 期刊: Anticancer Res 30
• 作者: Ariyoshi-Kishino K;Hashimoto K;Amano O;Saitoh J;Kochi M;Sakagami H
• 通讯作者: Sakagami H

歯科麻酔学7版

牙科麻醉学第七版

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臨床歯科麻酔学第4版

临床牙科麻醉学第四版

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• 通讯作者: 深山治久

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• 发表时间: 2011
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セボフルラン全身麻酔下でのペンタゾシンの聴性誘発電位(aepEX)とBIS値に及ぼす影響

七氟烷全身麻醉下喷他佐辛对听觉诱发电位（aepEX）和BIS值的影响

• 发表时间: 2011
• 作者: 塚本真規;小貫和之;小宅宏史;星島宏;竹内梨紗;長坂浩;土井克史;松本延幸
• 通讯作者: 松本延幸