Histological and immunohistochemical analysis of pulmonary hypoplastic lung and the effect of growth factor on lung growth in experimental fetal hypoplasia

实验性胎儿发育不全肺组织学和免疫组化分析及生长因子对肺生长的影响

• 批准号: 10671556
• 负责人: YOSHIMURA Shuichiro
• 金额: $ 1.6万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671556/
• 关键词: pulmonary hypoplasia; epidermal growth factor; immunohistochemistry; endocrine factor; growth factor; experiment study; rabbits model; hepatocyte growth factor; ARP; 免疫染色; GRP

Fetal pulmonary hypoplasia is a condition characterized by a decrease in the number of lung cells, airways, and alveoli, associated with a decrease in organ size and wight. The condition usually develops as a complication of prolonged severe oligohydramnios caused by bilateral renal agenesis, infantile polycystic kidneys, prolonged leakage of amniotic fluid or congenital diaphragmatic hernia(CDH).We demonstrated that pulmonary hypoplasia showed significant abnormalities of cellular growth and differentiation reveaved by immunohistochemical staining. But lung maturation in pulmonary hypoplasia was not significant compared with non-pulmonary hypoplasia.Our results suggested that EGF was associated with lung growth and maturation of human lung and that treatment of rabbit fetuses with hypoplastic lungs with EGF facilitated lung growth and development.

胎儿肺发育不全是一种以肺细胞、气道和肺泡数量减少为特征的疾病，与器官大小和重量减少相关。肺发育不全是由于双侧肾发育不全、婴儿型多囊肾、羊水长期渗漏或先天性膈疝（CDH）引起的羊水过少所致。结果提示：EGF与人肺发育不全胎兔肺的生长和成熟有关，EGF治疗肺发育不全胎兔可促进肺的生长和发育。

项目成果

Shuichiro Yoshimura,M.D.,Hideaki Masuzaki,M.D.,et,al: "Effect of Epidermal Growth Factor on lung growth in experimental fetal pulmonary hypoplasia"Early Human Development. 57. 61-69 (2000)

Shuichiro Yoshimura，医学博士，Hideaki Masuzaki，医学博士等人：“表皮生长因子对实验性胎儿肺发育不全中肺生长的影响”早期人类发育。

Shuichiro Yoshimura et al.: "Effect of epidermal growth factor on lung growth in experimental fetal pulmonary hypoplasia."Early Human Development. 57. 61-69 (2000)

Shuichiro Yoshimura 等人：“表皮生长因子对实验性胎儿肺发育不全肺生长的影响。”早期人类发育。

Shuichiro Yoshimura et al.: "Histological and immunohistochemical analysis of fetal hypoplastic lungs : preliminary study."ACTA MEDICA NAGASAKIENSIA. 45. 61-66 (2000)

Shuichiro Yoshimura 等人：“胎儿肺发育不良的组织学和免疫组织化学分析：初步研究。”ACTA MEDICA NAGASAKIENSIA。

Shuichiro Yoshimura et al.: "Effect of epidermal growth factor on lung growth in experimental fetal pulmonary hypoplasia"Early Human Development. 57. 61-69 (2000)

Shuichiro Yoshimura 等人：“表皮生长因子对实验性胎儿肺发育不全肺生长的影响”早期人类发育。

Shuichiro Yoshimura, et al.: "Histological and immunohistochemical analysis of fetal hypoplastic lungs : preliminary study"ACTA MEDICA NAGASAKIENSIA. 45. 61-66 (2000)

Shuichiro Yoshimura 等：“胎儿肺发育不良的组织学和免疫组织化学分析：初步研究”ACTA MEDICA NAGASAKIENSIA。