Study on G-CSF in ovulation, maintenance of pregnancy and other fields in obstetrics Gynecology

G-CSF在妇产科排卵、维持妊娠等领域的研究

• 批准号: 10671574
• 负责人: MAKINODA Satoru
• 金额: $ 2.05万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671574/
• 关键词: ovulation; Granulocyte colony-stimulating factor : G-CSF; cytokine; mRNA; maintenance of pregnancy; 顆粒球コロニー増殖因子(Granulocyte colony-stimulating factor:G-CSF); 妊娠; 予後; 顆粒状コロニー増殖因子(Granulocyte colony-stimulating factor : G-CSF)

1) The fluctuation of the serum cytokine concentration around the ovulationThe fluctuation of the serum concentrations of G-CSF and cytokines which had been reported close relation to ovulation was analyzed. As the result, no significant fluctuation could not be recognized in TNF-α, IL-1β, IL-6, GM-CSF and M-CSF around the ovulation. In contrast, the significant peak (40.12【plus-minus】2.02 pg/ml, M【plus-minus】SE) was observed at Day -5 〜 -1 in G-CSF. The more important role of G-CSF that other cytokines on ovulation was demonstrated.2) The comparison of serum and follicular fluid cytokine concentration just before the ovulationHigher concentration of the cytokines in the follicular fluid than in serum just before the ovulation was observed only in M-CSF, IL-6 and G-CSF. These follicular fluid/serum concentration ratio were 1.44, 1.69, 2.40, respectively. This phenomenon also clarified the participation of G-CSF in the mechanism of ovulation.3) The change of the G-CSF mRNA expression in … More follicular wall during human normal menstrual periodThe expression of G-CSF mRNA in follicular wall was examined quantitatively using Real-time quantitative RT-PCR method in patients who have received operation with informed consents. G-CSF mRNA levels were 15.3【plus-minus】0.9, 137.7【plus-minus】31.9, 14.8【plus-minus】3.7 and 5.3【plus-minus】3.5, in early follicular, late follicular, ovulatory and luteal phase respectively. The late follicular phase showed a significantly (p<0.00005) higher level than all other phases.The aim of the present study is to clarify the role of G-CSF in ovulation and maintenance of pregnancy.4) The examination on maternal serum G-CSF concentration as prediction method of prognosis in early stage of pregnancyThe serum concentration of G-CSF of 27 women who had Gestational Sac (GS) but no Fetal Heart Beat (FHB) was examined at first visit with informed consent. They were later classified into normal group (n=21) and abortion group (n=6) from pregnancy progress. Maternal serum G-CSF value in the normal group was 51.7【plus-minus】4.1 and distributed between 25〜105. In the abortion group, it was significantly (p<0.01) lower at 27.8【plus-minus】6.6 than the normal group. All objects ended in the abortion under 23.7 which was M-1.5S.D. of the normal group. It was clarified that G-CSF is useful for the prognostication in early stage of pregnancy.From the above result, the important role of G-CSF for ovulation and maintenance of pregnancy was clarified. We will continue the research on G-CSF including the field of Gynecology. Less

1)排卵前后血清细胞因子浓度的变化分析了与排卵密切相关的血清G-CSF和细胞因子浓度的变化。结果表明，TNF-α、IL-1β、IL-6、GM-CSF和M-CSF在排卵前后无明显波动。相反，在G-CSF中，在第-5天观察到显著峰（40.12[±]2.02 pg/ml，M[±]SE）。2）排卵前血清和卵泡液中细胞因子浓度的比较卵泡液中仅M-CSF、IL-6和G-CSF中细胞因子浓度高于排卵前血清。卵泡液/血清浓度比值分别为1.44、1.69、2.40。这一现象也进一步阐明了G-CSF参与排卵的机制。3）G-CSF mRNA表达的变化与卵巢组织中G-CSF的表达有关。 ...更多信息 应用实时荧光定量RT-PCR方法对知情同意手术患者卵泡壁G-CSF mRNA的表达进行了定量检测。G-CSFmRNA水平在卵泡早期、卵泡晚期、排卵期和黄体期分别为15.3 ± 0.9、137.7 ± 31.9、14.8 ± 3.7和5.3 ± 3.5。晚期卵泡期表现出明显的本研究旨在探讨G-CSF在排卵和维持妊娠中的作用。4）检测孕妇血清G-CSF浓度作为妊娠早期预测预后的一种方法。但在知情同意的情况下，首次访视时未检查胎心（FHB）。根据妊娠进展分为正常组（n=21）和流产组（n=6）。正常组孕妇血清G-CSF值为51.7[±]4.1，分布在25 ± 105之间。在流产组中，它显著（p<0.01）低于正常组，为27.8[±]6.6。所有对象均在23.7以下流产，即M-1.5S.D。的正常组。说明G-CSF在妊娠早期的促排卵和维持妊娠中的重要作用。我们将继续对G-CSF的研究，包括妇科领域的研究。少