Study on G-CSF in ovulation, maintenance of pregnancy and other fields in obstetrics Gynecology

1)排卵前后血清细胞因子浓度的波动分析了已报道的与排卵密切相关的血清G-CSF和细胞因子浓度的波动。结果表明，在排卵前后TNF-α、IL-1β、IL-6、GM-CSF和M-CSF均未见明显波动。G-CSF在第-5 ~ -1天达到40.12【正负】2.02 pg/ml， M【正负】SE。G-CSF对排卵的作用比其他细胞因子更重要。2)排卵前血清和卵泡液细胞因子浓度的比较卵泡液细胞因子浓度高于排卵前血清，仅在M-CSF、IL-6和G-CSF中可见。卵泡液/血清浓度比值分别为1.44、1.69、2.40。这一现象也阐明了G-CSF参与排卵的机制。3)正常月经期间卵泡壁G-CSF mRNA表达的变化采用Real-time定量RT-PCR法定量检测经知情同意接受手术的患者卵泡壁G-CSF mRNA的表达。卵泡早期、卵泡晚期、排卵期和黄体期G-CSF mRNA水平分别为15.3【正负】0.9、137.7【正负】31.9、14.8【正负】3.7和5.3【正负】3.5。卵泡晚期的水平显著高于其他各期（p<0.00005）。本研究的目的是阐明G-CSF在排卵和妊娠维持中的作用。4)检测母体血清G-CSF浓度作为妊娠早期预后的预测方法在知情同意的情况下，对27例有妊娠囊（GS）但无胎心搏（FHB）的妇女首次就诊时检测血清G-CSF浓度。根据妊娠进展分为正常组（n=21）和流产组（n=6）。正常组产妇血清G-CSF值为51.7【正负】4.1，分布在25 ~ 105之间。流产组为27.8【正负】6.6，明显低于正常组（p<0.01）。所有对象均以23.7岁以下流产结束，为M-1.5S.D。正常组的。结果表明，G-CSF对妊娠早期的预测有重要意义。以上结果阐明了G-CSF在排卵和妊娠维持中的重要作用。我们将继续研究G-CSF，包括妇科领域。少

1) The fluctuation of the serum cytokine concentration around the ovulationThe fluctuation of the serum concentrations of G-CSF and cytokines which had been reported close relation to ovulation was analyzed. As the result, no significant fluctuation could not be recognized in TNF-α, IL-1β, IL-6, GM-CSF and M-CSF around the ovulation. In contrast, the significant peak (40.12【plus-minus】2.02 pg/ml, M【plus-minus】SE) was observed at Day -5 〜 -1 in G-CSF. The more important role of G-CSF that other cytokines on ovulation was demonstrated.2) The comparison of serum and follicular fluid cytokine concentration just before the ovulationHigher concentration of the cytokines in the follicular fluid than in serum just before the ovulation was observed only in M-CSF, IL-6 and G-CSF. These follicular fluid/serum concentration ratio were 1.44, 1.69, 2.40, respectively. This phenomenon also clarified the participation of G-CSF in the mechanism of ovulation.3) The change of the G-CSF mRNA expression in … More follicular wall during human normal menstrual periodThe expression of G-CSF mRNA in follicular wall was examined quantitatively using Real-time quantitative RT-PCR method in patients who have received operation with informed consents. G-CSF mRNA levels were 15.3【plus-minus】0.9, 137.7【plus-minus】31.9, 14.8【plus-minus】3.7 and 5.3【plus-minus】3.5, in early follicular, late follicular, ovulatory and luteal phase respectively. The late follicular phase showed a significantly (p<0.00005) higher level than all other phases.The aim of the present study is to clarify the role of G-CSF in ovulation and maintenance of pregnancy.4) The examination on maternal serum G-CSF concentration as prediction method of prognosis in early stage of pregnancyThe serum concentration of G-CSF of 27 women who had Gestational Sac (GS) but no Fetal Heart Beat (FHB) was examined at first visit with informed consent. They were later classified into normal group (n=21) and abortion group (n=6) from pregnancy progress. Maternal serum G-CSF value in the normal group was 51.7【plus-minus】4.1 and distributed between 25〜105. In the abortion group, it was significantly (p<0.01) lower at 27.8【plus-minus】6.6 than the normal group. All objects ended in the abortion under 23.7 which was M-1.5S.D. of the normal group. It was clarified that G-CSF is useful for the prognostication in early stage of pregnancy.From the above result, the important role of G-CSF for ovulation and maintenance of pregnancy was clarified. We will continue the research on G-CSF including the field of Gynecology. Less