Effect of heavy charged particles on the normal eye

重带电粒子对正常眼睛的影响

• 批准号: 10671632
• 负责人: MIZOTA Atsushi
• 金额: $ 2.11万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671632/
• 关键词: heavy charged particle; carbon ion; rat; retina; crystalline lens; electroretinogram; 網膜電図; 眼球

Objectives : Heavy charged carbon particles are one kind of radiation and have begun to be used as the treatment for certain kinds of malignant tumors. However, this treatment has never been applied on the eye. A trial study of its irradiation on the orbital malignant tumors has just started in Japan. Since few reports are available about its influence on the retina, we studied the influence of heavy charged particles irradiation on the retina and crystalline lens.Methods : Wister rats (8 week-old, weighting about 150g) were used in this study . Right eyes were exposed to heavy charged carbon (ィイD112ィエD1C) particles irradiation in 5 different single doses (1, 2, 4, 8 and 16Gy) under general anesthesia with ketamine, xylazine and urethane. Six animals without irradiation were used as controls. The opacity of crystalline lens were evaluated with a surgical microscope and flash ERGs were recorded to evaluate retinal function at 1, 3 and 6 months after the irradiation.Results : At 1 month … More after the irradiation rats irradiated 8 and 16Gy showed significantly high incidence of cataract, and at 3 months all mice irradiated 16 Gy showed mature cataract. At 1 and 3 months, there were no significant changes in the amplitude of the a-waves in ERGs in any dose, but the amplitude of b-wave decreased significantly in the rats irradiated 16 Gy. At 6 months after the irradiation, the rats irradiated 8 and 16 Gy showed statistically significant decrease of the amplitudes of b-waves, but the amplitude od a-wave remained normal. In the rats with lower doses, there were no significant changes.Conclusions : With single irradiation of 8 Gy and 16 Gy cause cataract shortly after the irradiation. The retinae were not damaged with low doses (1, 2, and 4Gy) of heavy charged particles. But high dose (8 and 16Gy) caused retinal functional damage in a long term, although it has no influence in a short term. Our results could provide important information to apply the ィイD112ィエD1C particles irradiation on the eye clinically. Less

目的：重电荷碳粒子是一种辐射，已开始用于某些恶性肿瘤的治疗。然而，这种疗法从未被应用于眼睛。其照射治疗眼眶恶性肿瘤的试验研究在日本才刚刚开始。由于对视网膜影响的报道很少，我们研究了重电荷粒子照射对视网膜和晶状体的影响。方法：Wister大鼠(8周龄，体重约150g)。在氯胺酮、赛拉津和乌拉坦全身麻醉下，右眼接受重电荷碳(ィイD112ィエD1C)粒子照射，分5个单次剂量(1、2、4、8和16Gy5次)。6只未接受照射的动物作为对照。术后1、3、6个月在手术显微镜下观察晶状体混浊程度，并记录闪光ERG以评价视网膜功能。结果：1个月…照射后，8、16Gy组大鼠白内障发生率明显升高，3个月时16Gy组小鼠均出现成熟性白内障。照射后1个月和3个月时，各剂量组大鼠ERG的a波振幅无明显变化，而16Gy射线照射组的b波振幅显著降低。照射后6个月，照射8和16Gyb波的波幅显著降低，a波的波幅无明显变化。结论：8Gy1次照射和16Gy1次照射可在照射后即刻引起白内障。低剂量(1、2、4Gy重带电粒子)对视网膜无损伤。但是，高剂量(8和16Gy射线)虽然在短期内没有影响，但在长期内会导致视网膜功能损害。本研究结果可为临床应用ィイD112ィエD1C粒子照射眼部提供重要信息。较少

项目成果

Kiyokawa M, Mizota A, et al.: "Pattern visual evoked cortical potentials in patients with toxic optic neuropathy caused by toluene abuse"Jpn J Qphthalmol. 43. 438-442 (1999)

Kiyokawa M、Mizota A 等人：“甲苯滥用引起的中毒性视神经病变患者的视觉诱发皮层电位模式”Jpn J Q Phutmol。

Li S, Mizota A, Adachi-Usami E: "Effects of intravitreal injection of botulinum toxin on the electroretinogram of rats."Ophthalmic Res. 31. 392-398 (1999)

Li S、Mizota A、Adachi-Usami E：“玻璃体内注射肉毒杆菌毒素对大鼠视网膜电图的影响。”眼科研究。

Ishikawa K, Adachi-Usami E, Mizota A: "Relationship between visual field loss and maximal combined electroretinographic responses on retinitis pigmentosa : comparison among genetically different types."Chibaigaku. 75. 145-150 (1999)

Ishikawa K、Adachi-Usami E、Mizota A：“色素性视网膜炎的视野丧失与最大联合视网膜电图反应之间的关系：遗传不同类型之间的比较。”Chibaigaku。

Lis,Mizota A et al.: "Alterations of the electroretinogram by intravitreal kainic acid the rat"Jpn J Ophthalmol. 43. 495-501 (1999)

Lis，Mizota A 等人：“大鼠玻璃体内红藻氨酸对视网膜电图的改变”Jpn J Ophamol。

Lis,Mizota A et al.: "Effects of intravitreal injection of botulinum toxin on the electroretinogram of rats"Ophthalmic Res. 31. 392-398 (1999)

Lis，Mizota A 等人：“玻璃体内注射肉毒杆菌毒素对大鼠视网膜电图的影响”眼科研究。