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STUDY ON ROLE OF ALDEHYDE IN OXIDATIVE REACTION IN RETINA IN DUCED BY OXIDATIVE STRESS

醛在氧化应激引起的视网膜氧化反应中的作用研究

基本信息

批准号：
10671641
负责人：
HIRAMITSU Tadahisa
金额：
$ 1.98万
依托单位：
HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

As the backround study to investigate the factors to influence on the oxidative reactions in the retina, the localization of glutathione peroxidase (GSH-PO) in Wistar rats and Royal College of Surgeon rat was identified by the immuno-histochemical method. It was found that the degeneration of rod outer segment causes the dysfunction of the mitochondria in inner segment, resulting in the migration of GSH-PO from the innner segment to degraded rod outer segment.In the experimental protocol (planned for 1999) to investigate on the formation of aldehyde in RPE cells by illumination, the cell viability was investigated in cultured RPE cell with addition of linoleic acid or linoleic acid hydroperoxide. It was found that fluorescent lamp causes the oxygen species mediated cell injuries to RPE cells.In the experimental protocols (planned for 1998 and 1999) to investigate the formation of aldehydes and fluorescein products (RFP) derived from the iron-induced lipid peroxides, it was found that the formation of RFP increased gradually with the incubation time with the presence of albumin and HNE increased dramatically at early time of incubation and decreased after the time lapse. The formation of glutathione conjugates with HNE also was observed.From these results, the new findings as follos were obtained. The formation of lipofuscin like fluorescein products are related to the formation of aldehydes such as HNE and the diseases in which lipid peroxides are implicated may have an etiological relation with amount of aldehydes to be very harmful. Glutathione may be useful in the reduction of aldehydes formation derived from lipid peroxides.

作为探讨视网膜氧化反应影响因素的背景研究，本研究采用免疫组织化学方法对Wistar大鼠和皇家外科学院大鼠视网膜谷胱甘肽过氧化物酶（GSH-PO）进行了定位研究。结果发现，视杆外节的退变引起内节线粒体功能障碍，使GSH-PO由内节向退变的视杆外节迁移（计划于1999年）研究通过光照在RPE细胞中醛的形成，在添加亚油酸或亚油酸氢过氧化物的培养的RPE细胞中研究细胞活力。荧光灯对RPE细胞造成氧介导的细胞损伤（计划于1998年和1999年进行）研究铁诱导的脂质过氧化物产生的醛和荧光素产物（RFP）的形成，结果表明，白蛋白和HNE的存在使RFP的形成随孵育时间的延长而逐渐增加，HNE在孵育早期急剧增加的潜伏期，并随着时间的推移而减少。还观察到谷胱甘肽与HNE形成结合物。脂褐素样荧光素产物的形成与醛类如HNE的形成有关，并且其中涉及脂质过氧化物的疾病可能与醛类的量非常有害有病因学关系。谷氨酰胺可用于减少由脂质过氧化物衍生的醛形成。