Preventive Effects of Prostaglandin Inhibitors and Antioxidants on the Experimental Uveitis.

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1)Introduction : In order to study the preventive effect of anti-inflammatory drugs such as aspirin, a prostagalandin inhibitor, on the experimentally induced uveitis, the effects of aspirin vs steroid on endotoxin-induced experimental uveitis in the rabbits which may be related to the prostaglandins synthesis were studied.2)Methods : O. 1ml of endotoxin(20, mug)was intravitreally injected to the rabbit eye 16 hrs after the injection, the eye was enucleated and the iris was separated. As a indicator of the inflammation, myeloperoxidase(MPO)in the iris was determined. Before the intravitreal injections of endotoxin, aspirin DI-lysine(25mg/kg, 50mg/kg, 100mg/kg)or prednisolone(0.5mg/kg, lmg/kg, 2mg/kg)were performed.3)Results : No activity of MPO in the iris of the eyes which received the intravitreal injection of normal saline solution was-found. MPO activity of the iris of the eyes treated with endotoxin increased highly as 2.9x10^<-2>U/Mg protein.MPO activity of the iris was inhibited by about 15% in the groups treated with the injections of more than 50mg/kg of aspirin and in that of more than lmg/kg of prednisolone. However, there was no significant Difference in the both groups.4)Conclusion : The preventive effect of aspirin on the endotoxin-induced uveitis could be demonstrated. This result suggests that prostaglandins may be involved in the endotoxin induced uveitis.Other results : Lipid peroxidation did not increase in the iris of the endotoxin- treated eye. MPO activity in the iris of S-antigen-induced uveitis is now under investigation.