Influence of soft laser irradiation on gene expression of EGF receptor and extracellular matrix in epithelial keratinocytes.

To clarify the influence of soft laser irradiation on gene expression of epidermal growth factor receptor(EGFR)and extra cellular matrix in epithelial cells were examined.Normal human epithelial keratinocytes(NHEK)were cultured in a24well microplate(5X lO I D13文件D1 cell/cm I D12文件D1)，and laser device was used Trnpl D(GaAlAs diode，905 nm wave length，max out put10W，average2.4 mW，energy density 1.25J/cm I D12文件D1(150sec.))(Yoshida，Japan)。NHEK were irradiated for 20 sec.From the bottom of plate after incubated for 48hrs.EGFR expression was investigated immunohistochemically and in situ hybridization.Fibronectin and laminin were mesured by ELISA.Also integrinα2β1，keratin and actin filament of NHEK were examined.After laser irradiation，proliferation of NHEK was increased，activity and gene expression of EGFR was accelerated.Fibronectin in the medium showed no significant difference compered with control，although laminin in the medium was decreased after laser irradiation。On the other hand，integrin expression was activated，also keratin and actin filaments of NHEK were developed upon laser irradiation.These results suggest that laser irradiation activate EGFR of NHEK，so cell proliferation may accelerate，and activation of integrin and extra cellular matrix expression by laser irradiation may promote both cell adhesion and cell differentiation。

To clarify the influence of soft laser irradiation on gene expression of epidermal growth factor receptor(EGFR) and extra cellular matrix in epithelial cells were examined. Normal human epithelial keratinocytes(NHEK) were cultured in a 24 well microplate(5 X lOィイD13ィエD1cell/cmィイD12ィエD1), and laser device was used Trnpl D(GaAlAs diode, 905nm wave length, max out put 10W, average 2.4mW, energy density 1.25J/cmィイD12ィエD1(150sec.))(Yoshida, Japan). NHEK were irradiated for 20 sec. From the bottom of plate after incubated for 48hrs. EGFR expression was investigated immunohistochemically and in situ hybridization. Fibronectin and laminin were mesured by ELISA. Also integrin α2β1, keratin and actin filament of NHEK were examined.After laser irradiation, proliferation of NHEK was increased, activity and gene expression of EGFR was accelerated. Fibronectin in the medium showed no significant difference compered with control, although laminin in the medium was decreased after laser irradiation. On the other hand, integrin expression was activated, also keratin and actin filaments of NHEK were developed upon laser irradiation.These results suggest that laser irradiation activate EGFR of NHEK, so cell proliferation may accelerate, and activation of integrin and extra cellular matrix expression by laser irradiation may promote both cell adhesion and cell differentiation.