权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Influence of soft laser irradiation on gene expression of EGF receptor and extracellular matrix in epithelial keratinocytes.

软激光照射对上皮角质形成细胞EGF受体和细胞外基质基因表达的影响

基本信息

批准号：
10671710
负责人：
YAMAMOTO Shigehisa
金额：
$ 2.05万
依托单位：
Ohu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671710/
关键词：
soft laser epithelial cell EGF receptor in vitro extra cellular matrix

项目摘要

To clarify the influence of soft laser irradiation on gene expression of epidermal growth factor receptor(EGFR) and extra cellular matrix in epithelial cells were examined. Normal human epithelial keratinocytes(NHEK) were cultured in a 24 well microplate(5 X lOィイD13ィエD1cell/cmィイD12ィエD1), and laser device was used Trnpl D(GaAlAs diode, 905nm wave length, max out put 10W, average 2.4mW, energy density 1.25J/cmィイD12ィエD1(150sec.))(Yoshida, Japan). NHEK were irradiated for 20 sec. From the bottom of plate after incubated for 48hrs. EGFR expression was investigated immunohistochemically and in situ hybridization. Fibronectin and laminin were mesured by ELISA. Also integrin α2β1, keratin and actin filament of NHEK were examined.After laser irradiation, proliferation of NHEK was increased, activity and gene expression of EGFR was accelerated. Fibronectin in the medium showed no significant difference compered with control, although laminin in the medium was decreased after laser irradiation. On the other hand, integrin expression was activated, also keratin and actin filaments of NHEK were developed upon laser irradiation.These results suggest that laser irradiation activate EGFR of NHEK, so cell proliferation may accelerate, and activation of integrin and extra cellular matrix expression by laser irradiation may promote both cell adhesion and cell differentiation.

为探讨软激光照射对上皮细胞表皮生长因子受体（EGFR）基因表达及细胞外基质的影响。正常人表皮角质形成细胞（NHEK）在24孔板中培养（5 × 10 μ g/cm × D13 μ g/D12 μ g/D11），激光装置为Trnp 1D（GaAlAs二极管，波长905 nm，最大输出功率10 W，平均功率2.4mW，能量密度1.25J/cm × D12 μ g/D11（150秒））。（Yoshida，日本）。将NHEK照射20秒。孵育48小时后从板底部开始。免疫组化和原位杂交检测EGFR表达。用ELISA法测定纤连蛋白和层粘连蛋白。激光照射后，NHEK细胞增殖增加，EGFR的活性和基因表达增加。激光照射后，培养液中的纤维连接蛋白含量与对照组相比无显著性差异，而层粘连蛋白含量则有所下降。激光照射后，整合素的表达被激活，角蛋白和肌动蛋白纤维也被激活，提示激光照射激活了NHEK的EGFR，从而促进了细胞增殖，激光照射激活整合素和细胞外基质的表达，可能促进了细胞的粘附和分化。