Influence of soft laser irradiation on gene expression of EGF receptor and extracellular matrix in epithelial keratinocytes.

为了澄清软激光辐射对表皮生长因子受体（EGFR）的基因表达和外皮细胞中的额外细胞矩阵的影响。普通人表皮角质细胞（NHEK）在24孔微板（5X lO型D13型D1细胞/cm型D12型D1）中培养，激光器使用Trnpl D（GaAlAs二极管，905纳米波长度，最大输出功率10 W，平均功率2.4 mW，能量密度1.25 J/cm型D12型D1（150秒））（Yoshida， Japan）。NHEK在20秒内辐射。从盘子的底部孵化后48小时。EGFR表达是一种受调查的免疫化学和原位杂交。纤维素和拉明被ELISA混合了。也是整合蛋白α2β1，NHEK的氯胺酮和活性膜被测试，激光辐射后，NHEK增加，EGFR的活性和基因表达被加速。纤维素在介质中显示出没有明显的差异，而在介质中的纤维素在激光辐射后被降解。在另一方面，整合蛋白表达已被激活，也是NHEK在激光辐射上开发的氯胺酮和活性薄膜。这些结果建议使用激光辐射激活NHEK的EGFR，因此细胞增加可以加速，以及通过激光辐射激活的整合蛋白和额外的细胞基质表达可以两个细胞粘附和细胞差异。

To clarify the influence of soft laser irradiation on gene expression of epidermal growth factor receptor(EGFR) and extra cellular matrix in epithelial cells were examined. Normal human epithelial keratinocytes(NHEK) were cultured in a 24 well microplate(5 X lOィイD13ィエD1cell/cmィイD12ィエD1), and laser device was used Trnpl D(GaAlAs diode, 905nm wave length, max out put 10W, average 2.4mW, energy density 1.25J/cmィイD12ィエD1(150sec.))(Yoshida, Japan). NHEK were irradiated for 20 sec. From the bottom of plate after incubated for 48hrs. EGFR expression was investigated immunohistochemically and in situ hybridization. Fibronectin and laminin were mesured by ELISA. Also integrin α2β1, keratin and actin filament of NHEK were examined.After laser irradiation, proliferation of NHEK was increased, activity and gene expression of EGFR was accelerated. Fibronectin in the medium showed no significant difference compered with control, although laminin in the medium was decreased after laser irradiation. On the other hand, integrin expression was activated, also keratin and actin filaments of NHEK were developed upon laser irradiation.These results suggest that laser irradiation activate EGFR of NHEK, so cell proliferation may accelerate, and activation of integrin and extra cellular matrix expression by laser irradiation may promote both cell adhesion and cell differentiation.