Alveolar Epithelial Cell Dysfunction Induced By Flavored E-Cigarette Aerosols

加味电子烟气雾剂引起的肺泡上皮细胞功能障碍

基本信息

批准号：
10770080
负责人：
Beata Kosmider
金额：
$ 15.85万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10770080
关键词：
Administrative Supplement Aerosols Alveolar Cell Death Cell Death Induction Cell Separation Centers for Disease Control and Prevention (U.S.)Cessation of life Cinnamon - dietary Computer Models DNA Damage Data Devices Distal Electronic cigarette Epithelial Cells Epithelium Exposure to Family Female Flavoring Food Fruit Functional disorder Gases Gender Goals Grant Histones Hot Spot Human Incidence Ingestion Inhalation Knowledge Location Lung Mitochondria Mitochondrial DNA National Institute of Environmental Health Sciences Nicotine Nuclear Outcome Parents Particulate Persons Phase Predisposition Production Proliferating Propylene Glycols Publishing Pulmonary Surfactants Reporting Research Respiration Respiratory Disease Risk Sample Size Sex Differences Structure Surface Tension Time Toxic effect Woman alveolar epithelium alveolar type II cell cell injury e-cigarette aerosols electronic cigarette use electronic liquid epithelial repair inflammatory lung disease injured airway lung health lung injury lung regeneration male men mitochondrial dysfunction mitochondrial metabolism response sex stem cells trend vaping vegetable glycerin

项目摘要

Abstract E-cigarette devices produce an aerosol from e-liquids containing flavorings, nicotine, and vehicle. Inhaled e- cigarette aerosol can reach the alveolar region. Alveolar type II (ATII) cells produce and secrete pulmonary surfactant, which decreases surface tension. They also have a stem cell potential, proliferate and repair the epithelium after damage leading to lung regeneration. Due to their energy-demanding functions and location in the lung, they depend highly on mitochondria. Mitochondrial DNA lacks histones that serve as a barrier against damaging factors, contributing to its susceptibility to damage. Mitochondrial dysfunction can lead to mitophagy and ATII cell death. Our preliminary results in the parent R01 grant and this application demonstrate the harmful effect of e-cigarette aerosols with fruit and dessert flavors on human primary ATII cells. The goal of this administrative supplement proposal is to clarify further the sex influences on human primary ATII cell dysfunction caused by exposure to flavored constituents and e-cigarette aerosols. In Aim 1, we will compare DNA damage in ATII cells exposed to flavored constituents and e-cigarette aerosols with fruit and dessert flavors. In Aim 2, we will define the impact of this exposure on mitochondrial function in ATII cells. Obtained results will define sex influences on the respiratory injury risk of flavored constituents and e-cigarette aerosols.

抽象的电子烟设备从含有调味剂，尼古丁和媒介物的电子液体中产生气溶胶。吸入E- 香烟气溶胶可以到达肺泡区域。牙槽II型（ATII）细胞产生并分泌肺部表面活性剂，可降低表面张力。它们还具有干细胞电位，增殖并修复损伤后的上皮导致肺部再生。由于它们的能量要求功能和位置肺，它们在很大程度上取决于线粒体。线粒体DNA缺少组蛋白，可作为屏障破坏因素，导致其损坏的敏感性。线粒体功能障碍可能导致线粒体和Atii细胞死亡。我们在父级R01赠款中的初步结果，该应用程序证明了有害用果实和甜点风味对人类原发性ATII细胞的电子烟气溶胶的影响。目标的目标行政补充建议是进一步阐明对人类初级ATII细胞功能障碍的性别影响由暴露于调味的成分和电子烟气溶胶引起的。在AIM 1中，我们将比较DNA损伤在Atii细胞中，暴露于带有水果和甜点口味的味道成分和电子烟气溶胶中。在AIM 2中，我们将定义这种暴露对ATII细胞中线粒体功能的影响。获得的结果将定义性对调味成分和电子烟气溶胶的呼吸损伤风险的影响。