A study of calcification mechanism of arteriosclerosis

动脉硬化钙化机制的研究

• 批准号: 10671718
• 负责人: YAMAMOTO Hirotsugu
• 金额: $ 2.05万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671718/
• 关键词: arteriosclerosis; crystal; calcification; histopathology; FT-IR; oxalate calcium; phosphate calcium; SEM; 舌動脈; 下歯槽動脈; 歯髄動脈; 病理組織学; 石灰沈着; 成分分析

Arteriosclerosis which consisted of lipid pigmentation, atheroma and calcification, is mainly occurred in the tunica intima. Although many studies concerning about pathogenesis of the lesion have been reported, the study of crystal formation has not been done extensively and conclusively, as yet. Therefore, the purpose of this study was to investigate the morphologic feature of the crystal in the arteriosclerosis from histopathological and biomineralogical viewpoints, and the following results were obtained.Histopathologically, the arteriosclerosis was composed of fibrous hypertrophy of the tunica intima with formation of atheroma, ulcer and calcification. From the findings using a polarization microscopy several pieces of crystal structure, which showed with high birefringence, were scattered in the area of arteriosclerosis. The morphological features indicated a bundle rosette, or fused appearance of the crystals. In addition, the crystals were negative or dyed black with von Kossa staining.From the analysis using Fourier transform infrared spectroscopy and scanning electron microscopy, the component of the crystal was thought to be calcium phosphate and/or calcium oxalate. The pathogenesis may have a relationship of oxalosis due to intestinal disorders or infection of major parasite.

动脉粥样硬化主要发生在图尼卡内膜，包括脂质沉着、粥样硬化和钙化。虽然有关其发病机制的研究已有很多报道，但对晶体形成的研究还没有广泛和明确的结果。本研究从组织病理学和生物矿物学的角度探讨了动脉硬化中晶体的形态学特征，结果表明：动脉硬化主要表现为内膜图尼卡纤维增生，并伴有粥样硬化、溃疡和钙化。从使用偏振显微镜的发现来看，在动脉硬化的区域中散布着几片具有高双折射的晶体结构。形态特征表明，束玫瑰花结，或融合外观的晶体。此外，通过von Kossa染色，晶体呈阴性或染成黑色，通过傅立叶变换红外光谱和扫描电子显微镜分析，认为晶体的成分为磷酸钙和/或草酸钙。其发病机制可能与肠道疾病引起的草酸盐中毒或主要寄生虫感染有关。

项目成果

本多豊彦,宇都宮忠彦: "上顎洞炎症性病変における異所的石灰化の病理組織学的検討"日大口腔科学. 26巻2号. 101-109 (2000)

本田丰彦、宇都宫忠彦：“上颌窦炎性病变异位钙化的组织病理学检查”日本大学口腔医学杂志，第 26 卷，第 2 期，101-109（2000 年）。

Emiko Matsumura et al.: "A study of characteristic crystals in arteriosclerosis"Nihon Univ.J.Oral Sci.. Vol.27 No.1. 80-84 (2001)

Emiko Matsumura 等：“动脉硬化特征晶体的研究”Nihon Univ.J.Oral Sci.. Vol.27 No.1。

秋元芳明,山本浩嗣 他: "Oral and maxillofacial radiology today"Elsevier. 499-501 615-617 (2000)

Yoshiaki Akimoto、Hirotsugu Yamamoto 等人：“当今口腔颌面放射学”Elsevier 499-501 615-617 (2000)。

松村恵美子,山本浩嗣 他: "動脈硬化症にみられた興味ある結晶構造物に関する一知見"日大口腔科学. 80-84 (2001)

Emiko Matsumura、Hirotsugu Yamamoto 等人：“深入了解动脉硬化中发现的有趣晶体结构”日本大学口腔医学 80-84 (2001)。

松村恵美子,山本浩嗣 他: "動脈硬化症にみられた興味ある結晶構造物に関する一知見"歯科基礎医学会雑誌. 42巻5号. 144 (2000)

Emiko Matsumura、Hirotsugu Yamamoto 等人：“深入了解动脉硬化中发现的有趣的晶体结构”，日本基础牙科医学会杂志，第 42 卷，第 5. 144 期（2000 年）。