Recovery of bone forming ability with bone morphogenetic protein from radiation injury

利用骨形态发生蛋白恢复辐射损伤的骨形成能力

• 批准号: 10671777
• 负责人: HACHISU Reiko
• 金额: $ 2.05万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671777/
• 关键词: Radiation effects; bone formation; osteoblasts; cell differentiation; bone morphogenetic protein; 細胞の分化; ラット; アルカリフォスファターゼ; 石灰化

Previous study showed that irradiation to rat osteoblast-like cells (ROB cells) retards growth and differentiation of the cells. In the osteoblast differentiation, bone-morphogenetic protein-2 (BMP-2) commits undifferentiated cells to osteo-chondrogenic lineage cells and promotes osteoblast maturation. In case of C2C12 myoblasts, addition of BMP-2 into the cell culture convert cell differentiation into osteoblasts. To assess the effects of BMP-2 on irradiated bone tissues, we examine the effects of BMP-2 in irradiated culture cells to induce osteoblast differentiation.In ROB cells, irradiation with 4, 8, 12 Gy decreased the number of calcified bone nodules formed on culture day 12. Addition of BMP-2 into the irradiated ROB cells with 4 and 8 Gy partially recovered the formation of bone-like nodules. In C2C12 cells, irradiation with 4 and 8 Gy decreased the number of myotybe formed in culture. With the addition of BMP-2, irradiation with 4 and 8 Gy did not affect the induction of osteoblastic phenotypes such as induction of ALP activity and production of osteocalcin in C2C12 cells.These results suggest that the addition of BMP-2 into irradiated cells recover the osteoblast differentiation from radiation damage and induce cell differentiation into osteoblasts from myoblasts. BMP-2 may be available to recover the bone formation after radiation injury. Further study is necessary to evaluat the effects of BMP-2 on irradiated tissues in vivo.

以往的研究表明，大鼠成骨样细胞（ROB细胞）受到辐射后，其生长和分化受到抑制。在成骨细胞分化过程中，骨形态发生蛋白-2（BMP-2）将未分化细胞定向分化为成骨软骨细胞，促进成骨细胞成熟。在C2 C12成肌细胞的情况下，向细胞培养物中添加BMP-2将细胞分化转化为成骨细胞。为了评估BMP-2对辐射骨组织的影响，我们研究了BMP-2在辐射培养细胞中诱导成骨细胞分化的作用，在ROB细胞中，4，8，12戈伊的辐射减少了培养第12天形成的钙化骨结节的数量。在4和8戈伊照射的ROB细胞中加入BMP-2可部分恢复骨样结节的形成。在C2 C12细胞中，用4和8戈伊照射减少了培养物中形成的肌型体的数量。加入BMP-2后，4和8戈伊照射对C2 C12细胞ALP活性和骨钙素的表达等成骨细胞表型的诱导没有影响，提示BMP-2可恢复辐射损伤后的成骨细胞分化，并诱导成肌细胞向成骨细胞分化。BMP-2可能对放射性骨损伤后的骨形成有促进作用。BMP-2对受照组织的影响有待进一步研究。