A study on tissue irritation of sodium hypochlorite paste

次氯酸钠糊剂的组织刺激性研究

• 批准号: 10671804
• 负责人: KATSUUMI Ichiroh
• 金额: $ 1.73万
• 依托单位: The The Nippon Dental University, School of Dentistry at Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671804/
• 关键词: sodium hypochlorite; paste; oganic substance dissolving agent; tissue irritation; chemical cleaning of root canal; root canal preparation

The purpose of the present study was to evaluate how the paste 10% NaCl (produced by adding an inorganic compound thickener, montmorillonite, on an experimental basis) gives irritation on oral mucosa and lower incisor pulp of rat. The evaluation was performed through the measurement of the level of histamine determined by fluorometric method and histological observation.When 10% NaOCl paste or 10% NaOCl liquid is given to rat oral mucosa to expert action for 30 minutes, exposure, vacuolation and hypertrophy of lamina propria were noted from one hour after the procedure, and this tendency persisted up to 48 hours thereafter. Also, appearance of inflammatory cells and dilation of blood vessel were found. There was no difference in the degree of histological changes between the paste and the liquid. In the paste group, the histamine level showed the maximum value one hour after the procedure, and the level decreased over time up to 48 hours after. There was almost no difference between the paste and liquid.On lower incisor of rat, a cavity of 5 mm in depth was formed under high-speed cutting and cooling. For 30 minutes, the 10% NaOCl paste and the 10% NaOCl liquid were kept into contact with the cavity, which reached dental pulp. Immediately after this procedure to 48 hours after the procedure, pathohistological examination of dental pulp and measurement of histamine level were performed. On dental pulp, vascular dilation and appearance of inflammatory cells were recongnized from immediately after the procedure. There was no distinct difference due to the action between the paste and the liquid. However, the histamine level in the pulp of the paste group increased immediately after the procedure and reached the maximum value. Also the liquid group showed the maximum value after the procedure. In both groups, a tendency to decrease was observed thereafter.

本研究的目的是评价10%NaCl糊剂（在实验基础上添加无机复合增稠剂蒙脱石制成）对大鼠口腔粘膜和下切牙牙髓的刺激性。通过荧光法测定组胺水平和组织学观察进行评价，将10%NaOCl糊剂或10%NaOCl液体给予大鼠口腔粘膜进行专门作用30分钟，从术后1小时开始观察到暴露、空泡化和固有层肥大，这种趋势持续至术后48小时。结果表明，该糊剂与液体相比，组织学改变程度无显著性差异。在糊剂组中，组胺水平在手术后1小时显示出最大值，并且水平在手术后48小时内随时间推移而下降。在高速切削和冷却条件下，在大鼠下切牙上形成一个深约5mm的洞。保持10%NaOCl糊剂和10%NaOCl液体与腔接触30分钟，腔到达牙髓。术后即刻至术后48小时，进行牙髓病理组织学检查和组胺水平测定。术后即刻观察牙髓血管扩张及炎症细胞的出现。由于糊剂和液体之间的作用，没有明显差异。而糊剂组牙髓中组胺含量在术后即刻即升高并达到最大值。液体组在手术后也显示出最大值。在两组中，此后均观察到下降趋势。