A study on tissue irritation of sodium hypochlorite paste

本研究的目的是评价10% NaCl（在实验基础上添加无机复合增稠剂蒙脱土制成）膏体对大鼠口腔黏膜和下门牙髓的刺激作用。通过荧光法测定组胺水平和组织学观察进行评价。将10% NaOCl膏体或10% NaOCl液给予大鼠口腔黏膜专家作用30分钟后，从1小时开始观察到暴露、空泡化和固有层肥大，这种趋势持续到48小时。同时发现炎症细胞的出现和血管扩张。膏体和液体的组织学改变程度无差异。在膏体组中，组胺水平在手术后一小时达到最高值，并随着时间的推移下降至48小时后。膏体和液体之间几乎没有区别。在大鼠下切牙高速切削和冷却下形成5 mm深的空腔。将10% NaOCl膏体和10% NaOCl液体与牙腔接触30分钟，达到牙髓。术后立即至术后48小时进行牙髓病理组织学检查和组胺水平测定。在牙髓上，血管扩张和炎症细胞的出现在手术后立即被识别出来。由于膏体和液体之间的作用，没有明显的差异。而膏体组牙髓组组胺含量在手术后立即升高，达到最高值。液体组也出现术后最高值。此后，两组患者均有下降趋势。

The purpose of the present study was to evaluate how the paste 10% NaCl (produced by adding an inorganic compound thickener, montmorillonite, on an experimental basis) gives irritation on oral mucosa and lower incisor pulp of rat. The evaluation was performed through the measurement of the level of histamine determined by fluorometric method and histological observation.When 10% NaOCl paste or 10% NaOCl liquid is given to rat oral mucosa to expert action for 30 minutes, exposure, vacuolation and hypertrophy of lamina propria were noted from one hour after the procedure, and this tendency persisted up to 48 hours thereafter. Also, appearance of inflammatory cells and dilation of blood vessel were found. There was no difference in the degree of histological changes between the paste and the liquid. In the paste group, the histamine level showed the maximum value one hour after the procedure, and the level decreased over time up to 48 hours after. There was almost no difference between the paste and liquid.On lower incisor of rat, a cavity of 5 mm in depth was formed under high-speed cutting and cooling. For 30 minutes, the 10% NaOCl paste and the 10% NaOCl liquid were kept into contact with the cavity, which reached dental pulp. Immediately after this procedure to 48 hours after the procedure, pathohistological examination of dental pulp and measurement of histamine level were performed. On dental pulp, vascular dilation and appearance of inflammatory cells were recongnized from immediately after the procedure. There was no distinct difference due to the action between the paste and the liquid. However, the histamine level in the pulp of the paste group increased immediately after the procedure and reached the maximum value. Also the liquid group showed the maximum value after the procedure. In both groups, a tendency to decrease was observed thereafter.