The response of mechano-receptor to electrical stimulation in alveolar bone cell

牙槽骨细胞机械感受器对电刺激的反应

• 批准号: 10671841
• 负责人: OHTSUKA Hideyuki
• 金额: $ 2.05万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671841/
• 关键词: electrical stimulation; Guided bone regeneration; poly-L lactic acid membrane; absorbable membrane; mechanical stress; mechano-receptor; alveolar bone cell; 生体内吸収性圧電フィルム; ポリL-乳酸フィルム

The purpose of this study was to investigate the effectiveness of the regeneration around artificial bone defects and extraction sockets using electrified membrane, and to compare the effectiveness of membrane to GTAM【○!R】. Electrified membranes were absorbable poly-L-lactic acid (PLLA) membrane and non- absorbable VDF/TrFE membrane.At first, Wis tar-strain male rats, ages 13 to 15 weeks, were used. A hole of 2.0 mm in diameter was drilled in the femoral diaphysis. The control group's, experimental site was covered with only soft tissue, Group A's with GTAM【○!R】, Group B's non-electrified PLA, Group C's and D's with PLLA membrane (Mw=10 X 1OィイD14ィエD1 or 30 X 10ィイD14ィエD1). The experimental periods were 2 and 4 weeks. Microscopic histopathological observations were made.Second experiment was to compare the effectiveness of two methods using GTAM【○!R】 and other using non-absorbable VDF/TrFE membrane as barrier membranes on bone regeneration around the extraction sockets. Six beagle dogs P3 and P4 were removed. Group A' s experiment site was covered with expanded-polytetrafluoroethylene, Group B' s was covered with VDF/TrFE membrane, and the control groups was covered with only mucoperiosteum flap. Experimental periods were 2, 4 and 6 weeks, histopathological and bone histomnorphomnetric observations were made.The electrified membrane groups showed more accelerated bone growth and bone maturity than did the GTAM【○!R】 groups in the early stages. Especially in the 30 X 10ィイD14ィエD1 Mw PLLA group, where a greater amount of bone tissue and increase of maturity was observed. Results of the GTAM【○!R】 groups were almost the same as time control group, and inferior to the electrified membrane groups through all experimental periods. PLLA is easily shaped to fit bone surface to maintain desired space. PLLA film might therefore be used in clinical application in guided bone regeneration.

本研究的目的是探讨使用通电膜在人工骨缺损和拔牙窝周围再生的有效性，并将膜的有效性与GTAM[○！R]。通电膜为可吸收聚L-乳酸（PLLA）膜和不可吸收VDF/TrFE膜。在股骨骨干上钻一个直径2.0 mm的孔。对照组仅用软组织覆盖实验部位，A组用GTAM[○！R]、B组的非带电PLA、C组和D组的具有PLLA膜（Mw=10 × 10 ↑ [2] D ↓ [14] D ↓ [1]或30 × 10 ↑ [2] D ↓ [14] D ↓ [1]）。实验期为2周和4周。第二个实验是用GTAM[○！R]和其他使用不可吸收的VDF/TrFE膜作为屏障膜对拔牙窝周围骨再生的研究。取出6只比格犬P3和P4。A组实验部位覆盖膨胀聚四氟乙烯，B组实验部位覆盖VDF/TrFE膜，对照组仅覆盖粘骨膜瓣。实验时间为2、4、6周，进行组织病理学和骨组织形态计量学观察，结果表明，通电膜组骨生长和骨成熟速度明显快于GTAM组[○！在早期阶段的R]组。特别是在30 X 10 μ g/L D14 μ g/L D1 Mw PLLA组中，观察到骨组织量更大，成熟度增加。GTAM[○！R]组与时间对照组基本相同，但在整个实验期间均低于通电膜组。PLLA易于塑形，以适应骨表面，从而保持所需的间隙。因此，PLLA膜可用于引导骨再生的临床应用。