Cytotoxicity of polymerization-initiators in dentistry and prevention with antioxodants

牙科聚合引发剂的细胞毒性和抗氧化剂的预防

• 批准号: 10671842
• 负责人: FUJISAWA S.
• 金额: $ 1.79万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671842/
• 关键词: polymerization initiators; antioxidants; eugenol; radical scavenging; polymer radicals; dimer; cytotoxicity; reactive oxgen species; 2-アリル-4-メトキシフェノール; フェノキシルラジカル; DPPHラジカル; オルトビスフェノール; ラジカル禁止効果; フェノールラジカル; アゾビスイソブチロニトリル; カップリング化

To clarify the cytotoxic activity of polymerization initiators in the resin system, the cytotoxicity of camphorquinone (CQ), benzyl and 9-fluorenone (9F) against human tooth pulp fibroblasts, gingival fibroblasts and human submandibular tumor cells were studied. The cytotoxicity declined in the order : 9F>>Benzyl>CQ.The polymerizability of triethyleneglycoldimethacrylate of CQ was the highest. Taken together, CQ was the best initiator. Radical scavenging effects of eugenol-related compounds in benzoyl peroxide-methyl methacrylate system were studied, indicating that the radical scavenging activity of dimers (i.e. bis-eugenol, bis-MMP, bis-BHA ns bis-BMP) was higher than that of monomers (eugenol, 2-methoxy-4-methylphenol (MMP), 2-t-butyl-4-methoxy phenol (BHA) and 2-t-butyl-4-methylphenol (BMP)). In contrast, the interaction activity of polymer radicals with monomers showed a higher activity than that of dimers and BMP was the most effective. In 2-ally-4-X-phenols, 2-allyl-4-methoxyphenol was the most effective.Antioxidants such as eugenol related compounds were converted themselves into phenoxyl radicals during scavenging radicals and in addition, reactve oxygen radicals were produced. They caused cytotoxicity via apoptosis induction.

为了阐明树脂体系中聚合引发剂的细胞毒性活性，研究了蒽醌（CQ）、苄基和9-芴酮（9 F）对人牙髓成纤维细胞、牙龈成纤维细胞和人下颌下肿瘤细胞的细胞毒性。细胞毒性大小顺序为：9 F>>苄基>CQ，CQ的三甘醇二甲基丙烯酸酯的聚合能力最高。综合考虑，CQ是最佳的引发剂。研究了丁香酚类化合物在过氧化苯甲酰-甲基丙烯酸甲酯体系中的清除自由基能力，结果表明，二聚体（即双丁香酚、双MMP、双BHA和双BMP）的清除自由基能力高于单体（丁香酚、2-甲氧基-4-甲基苯酚（MMP）、2-叔丁基-4-甲氧基苯酚（BHA）和2-叔丁基-4-甲基苯酚（BMP））。相反，聚合物自由基与单体的相互作用活性比二聚体的活性高，BMP是最有效的。在2-烯丙基-4-X-酚类化合物中，2-烯丙基-4-甲氧基苯酚的清除效果最好，丁香酚等抗氧化剂在清除自由基的过程中自身转化为苯氧基自由基，并产生活性氧自由基。它们通过凋亡诱导引起细胞毒性。

项目成果

Fujisawa 他4名: "Cytotoxicity of photosensitizers comphorquinone and 9-fluorenone with visible light irradiation on a human submandibular-duct cell line in vitro" Archives of Oral Biology. 43. 73-81 (1998)

Fujisawa 和其他 4 人：“体外可见光照射下光敏剂康佛醌和 9-芴酮对人下颌下导管细胞系的细胞毒性”口腔生物学档案 43. 73-81 (1998)。

Fujisawa 他8名: "Radical modulating and cytotoxic activity of synthesized eugenol-related compounds"Anticancer Res. 20. 2955-2960 (2000)

Fujisawa 等 8 人：“合成的丁子香酚相关化合物的自由基调节和细胞毒性活性”Anticancer Res. 20. 2955-2960 (2000)

Fujisawa S.他1名: "^1H-NMR spectroscopic studies of the interaction of thymol with phospholipid liposomes" Dent. Mater. J. 17・2. 139-147 (1998)

Fujisawa S.等人：“百里酚与磷脂脂质体相互作用的^1H-NMR光谱研究”Dent Mater. 17・2 (1998)。

Fujisawa 他2名: "Cytotoxicity of methyl methacrylate (MMA) and related compounds and their interaction with dipalmitoyl phosphatidylcholine (DPPC) liposomes as a mosletor biomembrance"Oral Diseases. 6. 215-221 (2000)

Fujisawa 和其他 2 人：“甲基丙烯酸甲酯 (MMA) 和相关化合物的细胞毒性及其与作为 mosletor 生物膜的二棕榈酰磷脂酰胆碱 (DPPC) 脂质体的相互作用”口腔疾病。

Fujisawa S 他8名: "Radical intensity and cytotoxicity of butylated hydroxyanisole and its orthobisphenol dimer"Anticancer Res. 19(513). 5947-5952 (1999)

Fujisawa S 等 8 人：“丁基化羟基茴香醚及其邻位双酚二聚体的自由基强度和细胞毒性”Anticancer Res 19(513) (1999)。