Cytotoxicity of polymerization-initiators in dentistry and prevention with antioxodants

为明确聚合引发剂在树脂体系中的细胞毒活性，研究了樟脑醌(CQ)、苯基和9-荧酮(9F)对人牙髓成纤维细胞、牙龈成纤维细胞和人颌下肿瘤细胞的细胞毒性。细胞毒性依次为：9F&GT；&GT；苄基&GT；CQ。总而言之，CQ是最好的引发剂。研究了丁香酚相关化合物在过氧化苯甲酰-甲基丙烯酸甲酯体系中的自由基清除作用，结果表明，二聚体(即双丁香酚、双基质、双BHA和双BMP)的清除活性高于单体化合物(丁香酚、2-甲氧基-4-甲基苯酚、2-叔丁基-4-甲氧基苯酚和2-叔丁基-4-甲基苯酚)。相反，聚合物自由基与单体的相互作用活性高于二聚体，其中BMP的作用效果最好。2-烯丙基-4-甲氧基苯酚中，2-烯丙基-4-甲氧基苯酚效果最好，在清除自由基的过程中，丁香酚类化合物等抗氧化剂自身转化为苯氧基自由基，并产生反应性氧自由基。它们通过诱导细胞凋亡而产生细胞毒作用。

To clarify the cytotoxic activity of polymerization initiators in the resin system, the cytotoxicity of camphorquinone (CQ), benzyl and 9-fluorenone (9F) against human tooth pulp fibroblasts, gingival fibroblasts and human submandibular tumor cells were studied. The cytotoxicity declined in the order : 9F>>Benzyl>CQ.The polymerizability of triethyleneglycoldimethacrylate of CQ was the highest. Taken together, CQ was the best initiator. Radical scavenging effects of eugenol-related compounds in benzoyl peroxide-methyl methacrylate system were studied, indicating that the radical scavenging activity of dimers (i.e. bis-eugenol, bis-MMP, bis-BHA ns bis-BMP) was higher than that of monomers (eugenol, 2-methoxy-4-methylphenol (MMP), 2-t-butyl-4-methoxy phenol (BHA) and 2-t-butyl-4-methylphenol (BMP)). In contrast, the interaction activity of polymer radicals with monomers showed a higher activity than that of dimers and BMP was the most effective. In 2-ally-4-X-phenols, 2-allyl-4-methoxyphenol was the most effective.Antioxidants such as eugenol related compounds were converted themselves into phenoxyl radicals during scavenging radicals and in addition, reactve oxygen radicals were produced. They caused cytotoxicity via apoptosis induction.