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Study on pathogenesis of inveterate oral mucous membrane diseases : Investigation of superoxide anion generation from peripheral blood neutrophils and various plasma cytokine levels

顽固性口腔粘膜疾病发病机制的研究：外周血中性粒细胞产生的超氧阴离子和各种血浆细胞因子水平的研究

基本信息

批准号：
10671878
负责人：
HARADA Toshiro
金额：
$ 2.05万
依托单位：
Shimane Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

The purpose of this study was to investigate the pathogenesis of various oral membrane diseases by examining the effects of biscoclaurine alkaloids (BA ; cepharanthin^<【○!R】>) on clinical symptoms and plasma cytokine levels, superoxide anion (O_2^-) generation from peripheral blood neutrophils in patients.O_2^- generation was measured in terms of cytochrome C reduction, and plasma cytokine level was measured by enzyme-linked immunosorbent assay at Nihon-Igaku-Rinshokensa-Kenkyusho (Izumo City, Japan). The results were as follows :(1) The symptoms in oral lichen planus (OLP), recurrent aphtha and radiation-induced oral mucositis were improved by the administration of BA.(2)BA effectively reduced the O_2^- generation in OLP.Both the post-treatment period level of O_2^- generation, and the degree of clinical symptoms in patients with OLP were significantly lower (p<0.02) compared to those of the pre-treatment period.(3) A decreasing tendency in plasma TNF-α and IL-β levels according to the clinical courses of the patients with OLP was observed when the clinical symptoms were improved.These results suggest that the overproduction of O_2^- from peripheral blood neutrophils and various plasma cytokines such as TNF-α and IL-β may be closely related to pathogenesis of OLP.However, the pathogenesis of the other diseases such as recurrent aphtha, leukoplakia, and radiation-induced oral mucositis is still a theme of this research.

本研究的目的是通过观察双双鱼碱生物碱（BA; cephanthin）在口腔粘膜疾病中的作用，探讨其发病机制。[R] >)对患者临床症状及血浆细胞因子水平、外周血中性粒细胞超氧阴离子（O_2^-）生成的影响。在Nihon-Igaku-Rinshokensa-Kenkyusho（出云市，日本）采用酶联免疫吸附法测定O_2^-生成量和血浆细胞因子水平。结果表明：(1)BA可改善口腔扁平苔藓（OLP）、复发性口疮和放射性口腔黏膜炎的症状。(2)BA有效地减少了OLP中O_2^-的生成。与治疗前相比，治疗后患者的O_2^-生成水平和临床症状程度均显著降低（p<0.02）。(3)随着临床症状的改善，血浆TNF-α和IL-β水平随OLP患者临床病程的不同呈下降趋势。提示外周血中性粒细胞过量生成O_2^-和血浆各种细胞因子如TNF-α和IL-β可能与OLP的发病密切相关。然而，复发性口疮、白斑、放射性口腔黏膜炎等其他疾病的发病机制仍是本研究的主题。