Study on transplanted auricular cartilage after removal of articular disc or condylar head of the temporomandibular joint

颞下颌关节关节盘或髁突头切除后移植耳软骨的研究

• 批准号: 10671877
• 负责人: YOSHIMURA Yasuro
• 金额: $ 1.92万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671877/
• 关键词: TMJ (Temporomandibular joint); Condylar head removal; Auricular cartilage transplantation; Cytokines; 治癒過程における軟骨細胞

This study was done to investigate the relationship between occlusal impairment and loss of vertical dimension due to condylar removal, and to investigate the use autologous auricular cartilage for prevention of the occlusal impairment.We used 102 adult male ICR mice in three separate experimental groups for 2 years : condylectomy only ; condylectomy plus auricular cartilage transplantation ; and, sham operation. The treatments were performed using microsurgical microscopy. The mice were sacrificed 38 to 191 days after the operations. We investigated their occlusal abnormalities by macroscopic examination. Morphological changes of transplanted auricular cartilage and the opposite temporomandibular joint (TMJ) were also studied by hematoxylin-eosin stain and immunohistochemical examinations.The following results were obtained :1. Macroscopic findings : Occlusal impairment was observed in at least 5 of the 49 mice which had been successfully treated. Their mandibular bones shifted to the … More operated side and incisal contact was lost completely. This change brought about an inability to feed naturally and thus the eventual death of the mouse.2. Histological findings : Transplanted auricles seemed to change their positions because of a lack of fixation and mandibular movement, that is they slipped out laterally from the TMJ in many cases.3. Immunohistochemical findings : Cells facing the articular cavity and located near the articular surface of the fossa and condyle showed positive reactions to transforming growth factors-β (TGF-β), while chondrocytes under them generally revealed negative staining for TGF-β. Transplanted auricular chondrocytes presented slight positiveness or negative for TGF-β.As for the fibroblast growth factor-2 (TGF-2), cells lining the articular cavity, the cells under them and chondrocytes were all positive for TGF-2 ; however, the first category showed the strongest staining of the 3 different cell categories. Transplanted auricular chondrocytes presented weak partial staining.Bone morphogenetic protein (BMP) was generally not observed in the above mentioned cells and auricular choudrocytes.Laminin was not demonstrated in any cells comprising the articular structures. Further, transplanted auricular cells showed no staining. Less

本研究采用102只成年雄性ICR小鼠，分为单纯髁突切除组、髁突切除+耳廓软骨移植组和假手术组，研究髁突切除后垂直方向的改变与咬合损伤的关系，并探讨自体耳廓软骨移植对咬合损伤的预防作用。使用显微外科显微镜进行治疗。术后38 ~ 191天处死小鼠。我们通过肉眼检查来调查他们的咬合异常。通过苏木精-伊红染色和免疫组织化学染色观察移植耳软骨及对侧颞下颌关节的形态学变化。肉眼观察结果：在成功治疗的49只小鼠中，至少有5只观察到咬合损伤。他们的下颌骨转移到 ...更多信息 手术侧和切缘接触完全丧失。这种变化导致小鼠无法自然进食，最终导致小鼠死亡。2.组织学结果：移植的耳廓由于缺乏固定和下颌运动而改变了位置，即在许多情况下从TMJ侧向滑出。免疫组化结果：朝向关节腔、位于关节窝和髁突关节面附近的细胞对TGF-β呈阳性反应，而其下方的软骨细胞对TGF-β呈阴性反应。移植耳廓软骨细胞TGF-β呈弱阳性或阴性，成纤维细胞生长因子2（fibroblast growth factor-2，TGF-2）、关节腔衬层细胞、衬层细胞及软骨细胞均呈阳性，但以衬层细胞染色最强。移植的耳廓软骨细胞呈弱的部分染色，上述细胞和耳廓软骨细胞中基本未观察到骨形态发生蛋白（BMP），构成关节结构的任何细胞中均未观察到层粘连蛋白。此外，移植的耳廓细胞未显示染色。少