The effect on the temporomandibular joint morphogenesis and its development by restriction of jaw movement in mice

限制颌运动对小鼠颞下颌关节形态发生及其发育的影响

• 批准号: 12671935
• 负责人: YOSHIMURA Yasuro
• 金额: $ 2.24万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671935/
• 关键词: Temporomandibular joint; Exo-utero development; Restriction of jaw movement; Growth factor; 成長因子; マウス顎関節組織形成; 開口制限; 子宮外あるいは子宮内発育

By the maxillo-mandibular fixation, no significant systemic growth retardation of the embryo was not detected. But at the temporomandibular portion of the mouth movement restriction, the border between the cartilage of the condylar head and the calcified bone in the neck became unclear and diffuse macroscopically.Deformed and disarranged cells were found in fee perichondrium of the condylar head, furthermore abnormal ossification was also observed between the proliferating and prehypertrophic zones and therefore discontinuity cell arrangement was characteristic. This finding suggested that jaw movement played an important role in the prenatal development of the temporomandibular joint. From the immunohistochemical staining and western blotting method of basic fibroblast growth factor (b-FGF) and its receptor (FGFR-3) revealed high stainability not in the superficial condylar chondrocytes but rather deeper proliferating and prehypertrophic zones at the embryonic day (E) 15.5, and E17.5. While in adults of both sex, b-FGF and FGFR-3 showed a high stainability in the superficial chondrocyte zone and gradually decreased towards to deeper zones. These molecules seemed to function in the mechanisms of calcification. Western blotting analysis of FGFR-3 in the normal growth showed a high stainability at the E 15.5, E 17.5 and at 11th day birth but in adult it revealed a weak stainability. B-FGF in the control group revealed a similar tendency but in adult at most little staining was observed.

通过上下颌固定，未检测到胚胎明显的全身生长迟缓。但在口腔运动受限的颞下颌部，髁头软骨与颈部钙化骨的边界变得不清晰，肉眼可见弥漫性弥漫。髁头软骨膜内可见细胞变形、排列紊乱，且增殖区和肥大前期之间也可见异常骨化，细胞排列不连续。 特征。这一发现表明下颌运动在颞下颌关节的产前发育中发挥着重要作用。碱性成纤维细胞生长因子 (b-FGF) 及其受体 (FGFR-3) 的免疫组织化学染色和蛋白质印迹方法显示，胚胎日 (E) 15.5 和 E17.5 的浅层髁软骨细胞没有高染色性，而是更深的增殖和肥大前期区域。而在成年男女中，b-FGF 和 FGFR-3 在浅表软骨细胞区显示出高染色性，并向更深的区域逐渐降低。这些分子似乎在钙化机制中发挥作用。正常生长中的 FGFR-3 的蛋白质印迹分析显示，在 E 15.5、E 17.5 和出生第 11 天时具有较高的染色性，但在成人中，其染色性较弱。对照组中的B-FGF也表现出类似的趋势，但在成人中最多观察到很少的染色。