Study on changes in the projection manner of myelinated fibers following transection of the trigeminal nerve.

三叉神经横断后有髓纤维投射方式变化的研究

• 批准号: 10671892
• 负责人: NISHIMORI Toshikazu
• 金额: $ 2.37万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671892/
• 关键词: infraorbital nerve; myelinated fiber; trigeminal nucleus caudalis; sciatic nerve; nerve transection; lumbar spinal cord; c-Fos; glutamate receptor; Zif; 268; 脊髄後角; グルタメイト; MK-801; CNQX; 眼下窩神経; FOSタンパク質; 非NMDA型受容体; 三叉神経; CB-HRP; 三叉神経知覚核

The projection manner of myelinated primary affferents, the rat infraorbital nerve and lingual nerve, into the trigeminal nuclear complex was investigated histochemically at 1998. These myelinated fibers terminated in the main sensory nucleus, nucleus oralis, nucleus interporalis and the lamina I and laminae III-V of the nucleus caudalis. After the infraorbital nerve was transected, the projection fashion of myelinated fibers was changed in the trigeminal nucleus caudalis one week after this treatment. Although myelinated fibers do not terminate basically in lamina II of naive rats, a new projection of this myelinated fiber was observed in lamina II, suggesting that transection of primary afferents produces changes in neural plasticity in the central nervous system.To clarify the mechanisms involved in neural plasticity after nerve transection, the expression of transcription factors, c-Fos and Zif/268, was investigated by transection of primary afferents during 1999-2000. The expressi … More on of these transcription factors was induced by both transection and exposure of the infraorbital nerve in the trigeminal nucleus caudalis, but the region expressing these proteins following the two treatments was closely similar to each other in this nucleus, indicating that it is difficult to distinguish neurons expressing these proteins according to transection or exposure of the infraorbital nerve. On the other hand, transection of the sciatic nerve produced the expression of these proteins throughout laminae I/II of the dorsal horn at the 4 and 5th levels of the lumbar spinal cord, while the induction of these proteins by exposure of this nerve was restricted in the lateral one third of this lamina. The number of neurons expressed in this lateral one third of this lamina after nerve transection was closely similar to that derived from exposure of this nerve, indicating that the expression of these proteins induced in this region following transection of the sciatic nerve is derived from exposure of this nerve, while the expression of these proteins at the medial two third of this lamina is derived from nerve transection. This result suggested that it is able to investigate the mechanisms involved in the expression of these proteins induced by nerve transection. First, the effects of pretreatment with glutamate receptor antagonists on the expression of these proteins following transection of the sciatic nerve were investigated. The expression of these proteins in this lamina following nerve transection was reduced by this preteatment, but the effects were restricted in the lateral one third of this lamima whereas no effect was found in the medial two third of this region, suggesting that there may be considerable differences in the involvement of glutamate receptors between tissue injury following nerve exposure and nerve transection. Less

作者于1998年用组织化学方法研究了大鼠眶下神经和舌神经向三叉神经核复合体的投射方式。这些有髓纤维终止于感觉主核、口核、孔间核和尾核的I层和III-V层。切断眶下神经后，1周后三叉神经尾侧核内有髓纤维投射方式发生改变。虽然有髓纤维基本上不终止于幼稚大鼠的板层II，但在板层II中观察到这种有髓纤维的新投射，这表明切断初级传入神经引起中枢神经系统中神经可塑性的变化。在1999-2000年期间通过切断初级传入纤维进行了研究。快递 ...更多信息 在三叉神经核尾侧，眶下神经的切断和暴露均诱导了这些转录因子中的一种，但在该核中，两种处理后表达这些蛋白的区域彼此非常相似，这表明很难根据眶下神经的切断或暴露来区分表达这些蛋白的神经元。另一方面，横断的坐骨神经产生的表达，这些蛋白质在整个板层I/II的背角在第4和第5水平的腰脊髓，而这些蛋白质的诱导暴露的神经被限制在外侧三分之一的这个板。神经横断后，在该椎板外侧三分之一处表达的神经元数量与暴露该神经所产生的神经元数量非常相似，这表明坐骨神经横断后，在该区域诱导的这些蛋白质的表达来源于暴露该神经，而该椎板内侧三分之二处的这些蛋白质的表达来源于神经横断。这一结果表明，它是能够调查的机制，在这些蛋白质的表达诱导神经横断。首先，研究了用谷氨酸受体拮抗剂预处理对坐骨神经横断后这些蛋白质表达的影响。这些蛋白质的表达在这个椎板神经切断后减少了这种preatment，但影响被限制在外侧三分之一的这个lamima，而没有影响被发现在内侧三分之二的这个区域，这表明可能有相当大的差异，在组织损伤神经暴露和神经切断后的谷氨酸受体之间的参与。少

项目成果

S.Sakoda: "Differential projection into the trigeminal sensory nuclear complex of primer of events in nervating oral and facial tissues in the rat"Jpa.J.Oral Biology. 40/5. 569-574 (1998)

S.Sakoda：“大鼠口腔和面部组织神经活动引物对三叉神经感觉核复合体的差异投影”Jpa.J.口腔生物学。

S.Sakoda: "Differential projection into the trigeminal sensory nuclear complex of primary afferents innervating oral and facial tissues in the rat."Japanese Journal of Oral Biology. 40/5. 569-574 (1998)

S.Sakoda：“对大鼠口腔和面部组织的初级传入神经的三叉神经感觉核复合体的差异投射。”日本口腔生物​​学杂志。

Sumio Sakoda: "Differential projection into the trigeminal sensory nuclear complex of Primary afferents innervaring oral and facial tissues in the rat" Japanese Journal of Oral Biology. 40巻5号. 569-574 (1998)

Sumio Sakoda：“大鼠口腔和面部组织的初级传入神经的三叉神经感觉核复合体的差异投射”，《日本口腔生物​​学杂志》，第 40 卷，第 5 期。569-574（1998 年）。