EXPRESSION OF PREPRODYNORPHIN mRNA IN THE RAT NUCLEUS CAUDALIS

前强啡肽原 mRNA 在大鼠尾核中的表达

• 批准号: 03807121
• 负责人: NISHIMORI Toshikazu
• 金额: $ 1.15万
• 依托单位: MIYAZAKI MEDICAL COLLEGE (1992)Hiroshima University (1991)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807121/
• 关键词: Dynorphin; Gene expression; Nucleus caudalis; Pain; In situ hybridization; 三叉神経尾側核; insituハイブリダイゼイション

The expression of preproenkephalin mRNA was examined using techniques for quantitative analysis of in situ hybridization in nucleus caudalis neurons following electrical stimulation of trigeminal primary afferents at the low and high intensities. Stimulation at both intensities principally enhanced the expression of this mRNA in laminae I and II neurons of the nucleus caudalis. The enhancement of the expression was found at one and two hours after the stimulation of high and low intensities, respectively, and was continued until 24 hour at the end of stimulations. Quantitative analysis of in situ hybridization revealed that the increment in the expression of this gene appears to be mainly due to an increase in the intensity of expression of this mRNA.Compared with our previous reports in which the expression of preproenkephalin mRNA was enhanced in a bimodial temporal pattern after stimulation at the low intensity of the primary afferents and this enhancement were mainly due to increase in the number of the positive neurons, the present result for the expression of preprodynorphin mRNA indicate that the expression of this mRNA in nucleus caudalis neurons are increaced in different manner from that of preproenkephalin mRNA following stimulation of trigeminal primary afferents, suggesting that in the nucleus caudalis the expression of two opioid peptide genes, preprodynorphin and preproenkephalin, are differentially regulated by primary afferents.

在低强度和高强度电刺激三叉神经初级传入后，使用尾核神经元原位杂交定量分析技术检查前脑啡肽原mRNA的表达。两种强度的刺激主要增强尾核 I 层和 II 层神经元中该 mRNA 的表达。分别在高强度刺激后1小时和低强度刺激后2小时发现表达增强，并持续到刺激结束24小时。原位杂交的定量分析表明，该基因表达量的增加似乎主要是由于该mRNA表达强度的增加。与我们之前的报道中前脑啡肽原mRNA的表达在初级传入神经低强度刺激后以双峰时间模式增强且这种增强主要是由于阳性神经元数量的增加相比，目前的表达结果 前强啡肽原 mRNA 的研究表明，在刺激三叉神经初级传入神经元后，尾核神经元中该 mRNA 的表达与前脑啡肽原 mRNA 的表达以不同的方式增加，这表明在尾核中，前强啡肽和前脑啡肽这两种阿片肽基因的表达受到初级传入神经的差异调节。

项目成果

西森 利数: "神経細胞における遺伝子発現の調節" 組織培養. 17. 253-258 (1991)

Toshikazu Nishimori：“神经元基因表达的调节”组织培养 17. 253-258 (1991)。

George R. Uhl: "Jun B, c-jun, jun-D and c-fos mRNA in nucleus caudalis neurons : rapid selective enhancement by afferent stimulation." Mo1. Brain Res.11. 133-141 (1991)

George R. Uhl：“尾核神经元中的 Jun B、c-jun、jun-D 和 c-fos mRNA：传入刺激的快速选择性增强。”

Uhl,R.G.: "Jun-B,c-jun,jun-D and c-fos mRNA in nucleus caudalis neurons:rapid selective enhancement by afferent stimulation" Molecular Brain Research. 11. 133-141 (1991)

Uhl，R.G.：“尾核神经元中的 Jun-B、c-jun、jun-D 和 c-fos mRNA：传入刺激的快速选择性增强”分子脑研究。

Suemune,S.: "Trigeminal nerve endings of lingual mucosa and musculate of the rat" Brain Research. 586. 162-165 (1992)

Suemune,S.：“大鼠舌粘膜和肌肉的三叉神经末梢”大脑研究。

Toshikazu Nishimori: "Control of gene expression in neurons" Soshikibaiyou. 17. 253-258 (1991)

Toshikazu Nishimori：“神经元基因表达的控制”Soshikibaiyou。