Search for Anti-inflammatory Principles from Medicinal Plants by Monitoring Nitric Oxide Production in Activated Macrophages

通过监测活化巨噬细胞中一氧化氮的产生来寻找药用植物的抗炎原理

基本信息

批准号：
10671981
负责人：
FUSHIYA Shinji
金额：
$ 2.11万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Search for anti-inflammatory principles from medicinal plants was carried out by monitoring suppressive effect against nitric oxide (NO) production in activated macrophages (Mφ). The result of this investigation is as follows:1. The AcOEt soluble portion of the methanol extract of Acer nikoense, a hepatoprotective Japanese folk medicine, suppressed the NO production in activated Mφ when orally treated, and (+)-rhododendrol was isolated as an active compound. Epi-Rhododendrin isolated from the n-BuOH soluble portion suppressed the NO production in vivo, too.2. The methanol extract of Cleome droserifolia, one of the Egyptian folk medicines, suppressed the NO production in vitro, and two flavonoids were isolated as active compounds. Their structures were determined as 5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone and 5,4'-dihydroxy-6,7,8,3',5'-pentamethoxyflavone, respectively.3 The methanol extract of Andrographis paniculata, an Indonesian anti-inflammatory crude drug, suppressed the NO production in vivo. The active compound was isolated and identified to be neoandrographolide.4. The methanol extract of Caesalpinia sappan, one of the Indonesian folk medicines, suppressed the NO production in vitro, and a chalcone was isolated as an active compound. The structure was determined as 4,4'-dihydroxy-3,2'-dimethoxychalcone.5. Febrifugine, the main alkaloidal constituent of Dichroa febrifuga, an anti-malarial Chinese crude drug, potentiated the NO production in activated Mφ in vivo, which is the reason of the anti-malarial activity.

通过监测激活巨噬细胞中一氧化氮（NO）产生的抑制作用（Mφ），搜索了医疗植物的抗炎原理。这项调查的结果如下：1。一种肝保护剂的日本民间药物的Acer Nikoense的甲醇提取物的Acoet固体部分抑制了口服治疗时活化的Mφ的NO产生，（+） - 杜鹃花被分离为活性化合物。从N-BuOH固体部分分离的Epi-Rhododendrin也抑制了体内的NO产生。2。埃及民间药物之一的克氏菌droserifolia的甲醇提取物在体外抑制了NO产生，并将两种类黄酮分离为活性化合物。它们的结构被确定为5,4'-二羟基-6,7,8,3'-四甲氧基氟氟酮和5,4'-dihydroxy-6,7,7,8,3'-3'-pentamethoxyflavone，3，雄性Paniculata的甲醇提取物，是印度尼西亚抗激素的抗药性抗药性，不受印度尼西亚的抗药性生产，viv viv viv viv viv viv viv viv viv viv viv viv viv viv viv viv viv viv。活性化合物被分离并确定为新摩菌素。4。印尼民间药品之一的凯撒皮萨彭（Caesalpinia Sappan）的甲醇提取物在体外抑制了NO产生，并且将Chalcone分离为活性化合物。该结构被确定为4,4'-二羟基-3,2'-二甲氧基甲酮5。 Febrifugine是一种抗疟疾的中国粗制药物Dichroa Febrifuga的主要生物碱构成，它在体内活化的Mφ中潜在的NO产生，这是抗癌活性的原因。