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A Study on the Mechanism of Spin Clearance in In vivo ESR Measurement

体内ESR测量中自旋间隙机理的研究

基本信息

批准号：
10672035
负责人：
ANZI Kazunori
金额：
$ 2.43万
依托单位：
National Institute of Radiological Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

[ィイD114ィエD1C] carbamoyl-PROXYL and [ィイD114ィエD1C] methoxycarbonyl-PROXYL ([ィイD114ィエD1C] MC-PROXYL) were synthesized using liquid ammonia and [ィイD114ィエD1C] acetone as starting materials. The autoradiography of rats using these probes clearly demonstrated that [ィイD114ィエD1C] MC-PROXYL quickly distributed to brain through blood-brain-barrier whereas [ィイD114ィエD1C]carbamoyl-PROXYL did not. [ィイD114ィエD1C]MC-PROXYL distributed more to liver. Three-dimensional ESR images of the brain of rats taken with MC-PROXYL and carbamoyl-PROXYL suggested that MC-PROXYL is useful to make an image of rat brain. Radio protective effect of these spin probes was measured. MC-PROXYL injected to C3H mice (10 weeks old, male) at a dose of 600 mg/kg prolonged the survival of mice or even rescued (20% of total) the mice irradiated with γ-ray at 8.7 Gy. This finding suggests that MC-PROXYL may interact with free radicals generated by X-irradiation and lose the stable spin of the MC-PROXYL, which may be one of the mechanisms of spin clearance observed in in vivo ESR measurements.

使用液氨和[ィイD114ィD1C]丙酮作为起始原料合成[ィイD114ィエD1C]氨基甲酰基-PROXYL和[ィイD114ィエD1C]甲氧基羰基-PROXYL([ィイD114ィエD1C]MC-PROXYL)。使用这些探针对大鼠进行的放射自显影清楚地表明，[ィイD114ィエD1C] MC-PROXYL 通过血脑屏障快速分布到大脑，而 [ィイD114ィエD1C]carbamoyl-PROXYL 则不然。 [ィイD114ィエD1C]MC-PROXYL更多地分布于肝脏。用 MC-PROXYL 和氨基甲酰基-PROXYL 拍摄的大鼠脑部三维 ESR 图像表明 MC-PROXYL 可用于制作大鼠脑部图像。测量了这些自旋探针的放射防护效果。 MC-PROXYL 以 600 mg/kg 的剂量注射到 C3H 小鼠（10 周龄，雄性）中，延长了小鼠的存活时间，甚至挽救了接受 8.7 Gy γ 射线照射的小鼠（占总数的 20%）。这一发现表明，MC-PROXYL 可能与 X 辐射产生的自由基相互作用，并失去 MC-PROXYL 的稳定自旋，这可能是体内 ESR 测量中观察到的自旋清除机制之一。