Study of the molecular mechanism of transactivation of PPAR

过氧化物酶体增殖激活受体（PPARs）是核受体超家族的转录因子。PPARa主要在脂肪酸高分解代谢率的组织中表达，而PPARg是脂肪组织选择性的，它通过调节脂肪生成关键基因的表达来触发脂肪细胞分化和脂质储存。PPARS作为配体依赖性转录因子，与RXR异源二聚化后，与特异性应答元件PPRE结合，从而调控靶基因的表达。ppar被许多配体激活。这些配体通过PPAR表现出配体特异性作用，表明配体PPAR需要特定的辅物配合物。此外，配体的合成被认为是由配体代谢酶严格控制的。因此，我们试图鉴定ppar和配体代谢酶的共激活因子。

Peroxisome proliferator-activated receptors (PPARs) are transcription factors belonging to the nuclear receptor super family. PPARa is predominantly expressed in tissues exhibiting high catabolic rates of fatty acids, while PPARg is adipose tissue selective where it triggers adipocyte differentiation' and lipid storage by regulating the expression of genes critical for adipogenesis. PPARS function as ligand-dependent transcription factors, which, upon heterodimerization with RXR, bind to specific response element termed PPRE, thus reguleting the expression of target genes. PPARs are activated by a number of ligands. These ligands exhibit the ligand-specific action through PPARs, suggesting that the liganded PPAR would reqruite a specific coacitator complex. Furthermore, the synthesis of ligands are thought to be strictly controled by ligand metabolic eazymes.Thus, we tried to identified coactivators for PPARs and ligand metabolic enzymes.