The anthropologic study for drug metabolic enzyme CYP2C19 polymor-phisom.

1，基因分型为 *1，*2。对CYP 2C 19基因 *3多态性进行Sma Ⅰ酶切和BamHI酶切。对109名健康志愿者进行了充分的知情同意，这些志愿者均在父母和祖父母处拥有家谱。定位度（located degree）是指来源于日本东北地区的封闭人群，每个家系成员得1分，非来源于东北地区的受试者得0分。3、对109名志愿者的10 ml血液样本进行校正。应用已建立的PCR-RFLP方法对109名健康志愿者的白色血细胞基因组DNA进行CYP 2C 19基因分型，其中49名受试者为0定位度，47名受试者为6定位度，13名受试者为其他定位度。CYP 2C 19基因型在0定位度受试者中为 *1*1者占40%，为 *1* 者占28%。而CYP 2C 19基因型在6个位点的受试者中有21%为 *1*1，45%为 *1*2。CYP 2C 19基因型在0 ~ 6个位点的分布不同。5、对以往报道的CYP 2C 19基因型分布进行了研究。结果表明，在蒙古人种中存在两类不同的CYP 2C 19基因型分布，即欧亚人种中 *1*1基因型占40%，*1*2基因型占20%，波利尼西亚人种中 *1*1基因型占20%，*1*2基因型占40%。在本研究中，日本人CYP 2C 19基因型有两种类型，分别来源于欧亚蒙古人种和波利尼西亚蒙古人种。这些结果为日本人种起源的研究提供了重要的证据。

1, Genotyping for *1, *2. *3 in CYP2C19 polymorphism were establish by Sma I digestion for *2 polymorphism and BamHI digestion for *3 polymorphism. Digestion fragments of PCR products were determined by gel electrophoresis.2, Enough informed consent was give to one-hundred nine healthy volunteers, who research owns family tree at parents and grandparents. Located degree, which means originated from Tohoku district in Japan as a closed population, were given one point for each family tree member, and were given zero point for subjects without originated Tohoku district. Total points of located degree in family tree were used value of experimental subjects.3, Ten milliliter of blood samples were corrected from 109 volunteers. The CYP2C19 genotypings were determined by previous established PCR-RFLP method using of genomic DNA extracted from white blood cell in 109 volunteers.The 49 experimental subjects had zero located degree, the 47 subjects had six located degree and 13 subjects classified the others. The CYP2C19 genotypes were obtained 40% of *1*1 and 28% of *1* in subjects of zero located degree. On the other hand, the CYP2C19 genotype were obtains 21% of *1*1 and 45% of *1*2 in subjects of six located degree. The different distribution of CYP2C19 genotype were observed between the group zero and six located degree.5, The distribution of genotypes for CYP2C19 in previous reports were researched. The results were suggested that there were two type of Mongoloid groups having different CYP2C19 genotypes distribution, which is population having 40% of *1*1 genotype versus 20% of *1*2 genotype originate from Eurasian mongoloids and population having 20% of *1*1 versus 40% of *1*2 genotype originated from Polynesian mongoloids. In the present our results, there is two type of Japanese population in CYP2C19 genotypes which are originated from Eurasia Mongoloids Polynesian Mongoloids. The our results propose very important evidence for study of Japanese race origin.