The analysis of a new tumor-associated antigen, TSP-1, in the gene and protein expression levels

凝血酶原蛋白-1(TSP-1)是一种分子量为45万的同量大分子，由活化的血小板释放，也可由多种类型的细胞合成，包括内皮细胞、单核细胞、巨噬细胞、成纤维细胞和各种肿瘤细胞。虽然已经报道了几种抗TSP-1的单抗，但只有少数报道描述了它们的详细特异性。在本研究中，我们制备了13种新的抗TSP-1单抗。其中6例为IgM型，4例为IgG1型，另有1例为IgG3型。组织化学和血清学鉴定表明，其中一些单抗可用于多种肿瘤的免疫组织化学研究和/或血清TSP-1水平的测定。另一方面，TSP-1是细胞外基质(ECM)的组成部分，是一种黏附分子，能够与各种游离大分子和多种细胞结合。虽然许多报道描述了细胞外基质蛋白的作用，但描述TSP-1对免疫细胞的影响的文献似乎很少。在本研究中，我们还研究了TSP-1在植物血凝素存在下对T细胞和抗原提呈细胞(APC)体外培养的影响，发现TSP-1可以促进APC的可溶性T细胞增殖和增殖，并促进APC分泌IL-10。

Thrombospodin-1 (TSP-1), a homotrimetric macromolecule with a molecular weight of 450,000, is released from activated platelets and also synthesized by many types of cells including endothelial cells, monocytes, macrophages, fibroblasts, and various tumor cells. Although several monoclonal antibodies (MAbs) to TSP-1 have been reported, only a few reports have described their detail specificity.In this study, we prepared 13 new monoclonal antibodies to TSP-1. Six of them were of the IgM variety, four were IgG1 and there were IgG3. Histochemical and serological characterization demonstrated that some of these MAbs are useful for immunohistochemical study and/or for determination or serum levels of TSP-1 in various cancers.On the other hand, TSP-1, a component of extracellular matrices (ECMs), is an adhesion molecule and capable of binding various free macromolecules and many types of cells. Although many reports described the effects of ECM proteins, there seem to be few publications describing the effects of TSP-1 on immune cells. In the present study, we also studied the influence of TSP-1 upon in vitro culture of T cells and antigen-presenting cells (APCs) in the presence of phytohemagglutinin and found that soluble T cell proliferation and proliferation and enhances IL-10 secretion by APCs.Taken together, the results obtained here indicate that TSP-1 may serve as a potential useful tumor maker and an immunosuppressive modulator possibly in inflammatory sites.