Study on the Usefulness of the Novel Tumor Marker MK-1 in the Diagnosis and Therapy of Cancer

用ELISA法检测MK-1，发现MK-1对某些恶性肿瘤有一定的诊断价值。人单克隆抗MK-1抗体M13-57显示了对MK-1表达细胞的抗体依赖性细胞介导的细胞毒性和补体依赖性细胞毒性，表明该抗体可用于基于抗体的癌症治疗。此外，由M13-57 scFv、CD 28和CD 3+组成的嵌合T细胞抗原受体在MK-1结合后诱导野生型T细胞受体样分子事件，表明这可能对癌症治疗有用。

Using the ELISA for MK-1, we found that MK-1 is useful for some malignant tumors. A human monoclonal anti-MK-1 antibody, M13-57, revealed antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity against MK-1-expressing cells, suggesting that this antibody may be useful for antibody-based therapy of cancer. Also, a chimeric T-cell antigen receptor consisting of M13-57 scFv, CD28 and CD3 ζ induced a wild-type T-cell receptor-like molecular event upon MK-1 binding, suggesting that this may be useful for cancer therapy.