EFFECT OF EXERCISE IN RATS UNDER DIFFERENT NUTRITIONAL STATE OF VITAMIN B6

不同维生素B6营养状态下运动对大鼠的影响

基本信息

批准号：
10680143
负责人：
OKADA Mitsuko
金额：
$ 2.05万
依托单位：
Naruto University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Over feeding against energy expenditure results in obesity and obesity causes many other diseases, such se diabetes, hypertension and so on. Exercise is recommended as a manner increasing energy expenditure to maintain normal body weight. Glycogen is an important source of glucose for energy substrate metabolism and ATP generation. Energy necessary for muscle contraction is supplied from glucose directly or indirectly, of which one is from glycogen by glycogen phosphorylase and the other is glucose from amino acids by aminotransferase. Both glycogen phosphorylase and aminotransferase are required vitamin B6 as the coenzyme. So we started to study how exercise is related to B6 requirement.Young male rats were used for the experiment, and this study is consisted of 2 experiments. In the experiment 1, rats were fed B6 free diet and trained 60min swimming every other day for 4 weeks. In the experiment 2, rats were fed B6 containing diet (1.45mg/kg diet) and trained 60min swimming every day for 3 weeks. These rats were killed, and blood and tissues were used for measurement of B6 content and B6-dependent enzyme activities. Results obtained are mainly as follows; Exercise resulted in increased metabolism of glycogen and amino acids in the tissues, especially gastrocnemius muscle. Abnormal amino acid metabolism in the B6-deficient rats was rendered to the normal level. Exercise was also useful to spare B6 consumption by accumulation of B6 in the tissues and by decreasing of B6 excretion into the urine. Totally B6 requirement should be increased in the exercised rat.

过度反对能量消耗会导致肥胖和肥胖导致许多其他疾病，例如糖尿病，高血压等。建议运动作为增加能量消耗以保持正常体重的方式。糖原是能量底物代谢和ATP产生的葡萄糖的重要来源。肌肉收缩所需的能量是直接或间接从葡萄糖提供的，其中一种是通过糖原磷酸化酶从糖原中提供的，另一种是通过氨基转移酶来自氨基酸的葡萄糖。糖原磷酸化酶和氨基转移酶都必须维生素B6作为辅酶。因此，我们开始研究运动与B6的需求如何相关的。雄性大鼠用于实验，这项研究由2个实验组成。在实验1中，大鼠免费饮食喂食，每隔一天训练60分钟游泳4周。在实验2中，将大鼠喂入含有饮食的B6（1.45mg/kg饮食），并每天训练60分钟游泳3周。这些大鼠被杀死，血液和组织用于测量B6含量和B6依赖性酶活性。获得的结果主要如下；运动导致组织中糖原和氨基酸的代谢增加，尤其是甲壳虫肌肉。 B6缺陷大鼠中异常的氨基酸代谢被渲染到正常水平。通过B6在组织中的积累以及将B6排泄量减少到尿液中，运动也可用于免除B6的消耗。在行使的老鼠中应完全增加B6的要求。