Influence of NiィイD12+ィエD1 and CoィイD12+ィエD1 on activity of microorganisms related to methanogenesis

NiD12+D1和CoD12+D1对产甲烷相关微生物活性的影响

• 批准号: 10680549
• 负责人: KIDA Kenji
• 金额: $ 1.73万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680549/
• 关键词: methane fermentation; aceticlastic methanogen; coenzyme; FィイD2420ィエD2; corrinoid; FィイD2430ィエD2; methanogenic pathway; メタン生成細菌; F430; 菌体活性; 代謝変換

The influence of NiィイD12+ィエD1 and CoィイD12+ィエD1 on methanogenic activity and the coenzymes involved in methanogenesis were investigated in an anaerobic continuous cultivation with synthetic wastewater containing acetate as the sole carbon source. Addition of NiィイD12+ィエD1 and CoィイD12+ィエD1 to the synthetic wastewater increased the concentrations of coenzymes corrinoids and FィイD2430ィエD2 in biomass. Their concentrations were approximately constant, being 0.55-0.62 and 0.56-0.67 nmol/g-VSS at a dilution rate of more than 0.12 dィイD1-1ィエD1. Methanogenic activity of biomass also increased with an increase in dilution rate. However, without addition of NiィイD12+ィエD1 and CoィイD12+ィエD1, corrinoids and FィイD2430ィエD2 were not detected in biomass and the methanogenic activity was zero at a dilution rate of 0.025 dィイD1-1ィエD1. These results suggest that NiィイD12+ィエD1 and CoィイD12+ィエD1 accelerated the methane-producing reactions via increases of coenzymes coninoids and FィイD2430ィエD2.The effects of NiィイD12+ィエD … More 1 and CoィイD12+ィエD1 limitation on methanogenesis from acetate were investigated using a mesophilic anaerobic continuous cultivation. A significant inhibition in methanogenic activity and degradation rate of acetate in response to a decrease in coenzymes corrinoids and FィイD2430ィエD2 contents in biomass was observed with a decrease of metal ion concentrations added. An increase of coenzyme FィイD2420ィエD2 and improvement in the degradation rate of acetate was observed with a decrease in dilution rate under metal ion limitation. These results suggest that an alternative pathway from acetate to methane via syntrophic conversion by acetate oxidizing bacteria and hydrogenophilic methanogens occupied a considerable portion of the whole bioconversion at a low dilution rate under metal ion limitation. The populations of aceticlastic and hydrogenophilic methanogens estimated by the most probable number (MPN) method also supported this suggestion.A mesophilic anaerobic continuous cultivation with synthetic wastewater containing propionate and acetate (3 : 1 in molar ratio) was constructed. This cultivation is under stable conditions. We are now trying to investigate the mechanisms of anaerobic degradation of propionate. Less

以含醋酸酯的合成废水为唯一碳源进行厌氧连续培养，考察了NiィイD12+ィエD1和CoィイD12+ィエD1对产甲烷活性及产甲烷辅酶活性的影响。在模拟废水中添加Ni-ィイ-D12+ィエ-D1和Co-ィイ-D12+ィエ-1，可提高生物质中辅酶类化合物和F-ィイ-D2430-ィエ-D2的含量。当稀释率大于0.12dィイd1~(-1)ィエd1时，它们的浓度基本恒定，分别为0.55~0.62和0.56~0.67nmol/g-Vss。生物质的产甲烷活性也随着稀释率的增加而增加。而在不添加Ni-ィイ-D12+ィエ-1和Co-ィイ-D12+ィエ-1的情况下，菌体中未检测到柯拉宁类化合物和F-ィイ-D2430-ィエ-D2，在稀释0.025 dィイ-1-1-ィエ-1时，产甲烷活性为零。这些结果表明，NiィイD12+ィエD1和CoィイD12+ィエD1通过增加辅酶Coninoid和FィイD2430ィエD2来促进甲烷生成反应。NiィイD12+ィエD…的作用采用中温厌氧连续培养法研究了More-1和Co-ィイD12+ィエ-D1对乙酸乙酯产甲烷的限制作用。随着金属离子浓度的降低，菌体中辅酶Corrinoid和F-ィイD2430-ィエ-D2含量的降低对产甲烷活性和乙酸酯降解速率有明显的抑制作用。在金属离子限制下，随着稀释率的降低，辅酶FィイD2420ィエD2增加，醋酸酯降解率提高。这些结果表明，在金属离子限制下，乙酸盐氧化细菌和嗜氢产甲烷菌以较低的稀释率从乙酸盐转化为甲烷的交替途径占据了整个转化过程的相当大比例。用最可能数(MPN)法估算的产乙酸菌和产氢产甲烷菌的数量也支持这一观点。利用含有丙酸酯和醋酸酯(摩尔比为3：1)的合成废水构建了中温厌氧连续培养系统。这一栽培条件稳定。我们现在正试图研究丙酸厌氧降解的机理。较少

项目成果

木田建次 他: "酢酸資化性メタン生成細菌の潜在能と代謝変換" 平成10年度日本水環境学会シンポジウム講演集. 157-158 (1998)

Kenji Kida 等人：“乙酸同化产甲烷细菌的潜在潜力和代谢转化”1998 年日本水环境学会研讨会论文集 157-158 (1998)。

木田 建次: "メタン生成細菌の能力制御と代謝変換"日本生物工学会大会(平成11年度)講演要旨集. 46-46 (1999)

Kenji Kida：“产甲烷细菌的能力和代谢转化的控制”日本生物工程学会会议摘要集（1999 年）46-46（1999 年）。

木田建次: "メタン生成細菌の能力制御と代謝変換"日本生物工学会大会(平成11年度)講演要旨集. 46 (1999)

Kenji Kida：“产甲烷细菌的能力和代谢转化的控制”日本生物工程学会会议摘要集（1999）46（1999）。

木田建次: "メタン発酵の代謝経路とその変換"環境管理. 35巻・6号. 539-546 (1999)

Kenji Kida：“甲烷发酵代谢途径及其转化”，环境管理，第 35 卷，第 6 期，539-546。

Kenji Kida et al.: "Control of methanogenic activity and conversion of metabolic pathway of microorganisms related to methane fermentation"The programs of Soc. Biosci. Bioeng.. 46 (1999)

Kenji Kida等：“甲烷发酵相关微生物产甲烷活性的控制和代谢途径的转换”Soc的程序。