Influence of NiィイD12+ィエD1 and CoィイD12+ィエD1 on activity of microorganisms related to methanogenesis

The influence of Ni D12+ die D1 and Co di D12+ die D1 on methanogenic activity and The coenzymes involvedin methanogenesis were investigated in anaerobic continuous cultivation with synthetic wastewatercontaining acetate as the sole carbon source. Addition of Ni D12+ D1 and Co Ni D12+ D1 to thesynthetic wastewater increased the concentrations of coenzymes corrinoids and fd - 2430 - D2 inbiomass. Their concentrations were approximately constant，being 0.55-0.62 and 0.56-0.67 nmol/g-VSS at a dilution rate of more than 0.12 d维D1-1维D1.Methanogenic activity of biomass also increased with an increase in dilution rate. However，without addition of Ni D12+ D1 and Co Ni D12+ D1，corrinoids and fy D2430 D2 were not detected in biomass and the methanogenic activity was zero at adilution rate of 0.025 d -1 d -1 d -1 d -1 . These results suggest that Ni D12+ d -1 and Co - D12+ d -1accelerated the methan -producing reactions via increases of coenzymes coninoids andd24 - 30 D2.The effects of Ni D12+ Ni D12…D12+ D12 limitation on methanogenesis from acetate were investigated using amesophilic anaerobic continuous cultivation. A significant inhibition in methanogenic activity anddegradation rate of acetate in response to a decrease in coenzymes corrinoids and fd - 2430 - D2contents in biomass was observed with a decrease of metal ion concentrations added. An increase ofcoenzyme F - D2420 - D2 and improvement in the degradation rate of acetate was observed with adecrease in dial rate under metal ion limitation.这些选择是一个替代方案pathway from acetate to methane via syntrophic conversion by acetate oxidizing bacteria andhydrogenophilic methanogens occupied a considerable portion of the whole bioconversion at a lowdipulations under metal ion limitation. The populations of aceticlastic and hydrogenophilicmethanogens estimated by the most probable number (MPN) method also supported this suggestion.Amesophilic anaerobic continuous cultivation with synthetic wastewater containing propionate andacetate (3：1 in molar ratio) was constructed. This cultivation is under stable conditions. We are now trying toinvestigate the mechanisms of anaerobic degradation of propionate. Less

The influence of NiィイD12+ィエD1 and CoィイD12+ィエD1 on methanogenic activity and the coenzymes involved in methanogenesis were investigated in an anaerobic continuous cultivation with synthetic wastewater containing acetate as the sole carbon source. Addition of NiィイD12+ィエD1 and CoィイD12+ィエD1 to the synthetic wastewater increased the concentrations of coenzymes corrinoids and FィイD2430ィエD2 in biomass. Their concentrations were approximately constant, being 0.55-0.62 and 0.56-0.67 nmol/g-VSS at a dilution rate of more than 0.12 dィイD1-1ィエD1. Methanogenic activity of biomass also increased with an increase in dilution rate. However, without addition of NiィイD12+ィエD1 and CoィイD12+ィエD1, corrinoids and FィイD2430ィエD2 were not detected in biomass and the methanogenic activity was zero at a dilution rate of 0.025 dィイD1-1ィエD1. These results suggest that NiィイD12+ィエD1 and CoィイD12+ィエD1 accelerated the methane-producing reactions via increases of coenzymes coninoids and FィイD2430ィエD2.The effects of NiィイD12+ィエD … More 1 and CoィイD12+ィエD1 limitation on methanogenesis from acetate were investigated using a mesophilic anaerobic continuous cultivation. A significant inhibition in methanogenic activity and degradation rate of acetate in response to a decrease in coenzymes corrinoids and FィイD2430ィエD2 contents in biomass was observed with a decrease of metal ion concentrations added. An increase of coenzyme FィイD2420ィエD2 and improvement in the degradation rate of acetate was observed with a decrease in dilution rate under metal ion limitation. These results suggest that an alternative pathway from acetate to methane via syntrophic conversion by acetate oxidizing bacteria and hydrogenophilic methanogens occupied a considerable portion of the whole bioconversion at a low dilution rate under metal ion limitation. The populations of aceticlastic and hydrogenophilic methanogens estimated by the most probable number (MPN) method also supported this suggestion.A mesophilic anaerobic continuous cultivation with synthetic wastewater containing propionate and acetate (3 : 1 in molar ratio) was constructed. This cultivation is under stable conditions. We are now trying to investigate the mechanisms of anaerobic degradation of propionate. Less