天然ホルモン除去を含む家畜糞尿・生ごみ混合物の高効率サーマルリサイクル技術の開発

开发牲畜粪便/垃圾混合物的高效热回收技术，包括去除天然激素

• 批准号: 18580331
• 负责人: KIDA Kenji
• 金额: $ 2.46万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580331/
• 关键词: methane fermentation; cow manure waste; natural steroid hormone; ozone oxidation; microbial community; サーマルリサイクル; 資源循環システム

An efficient treatment process for screened cow manure waste, particularly for the degradation of natural steroid hormones, was developed. The first step in this process was a draw-and-fill process for thermophilic anaerobic digestion. After fourfold dilution with tap water, continuous feeding was performed for the aerobic treatment of the effluent from the anaerobic treatment. Batchwise ozone oxidation was then carried out for the degradation of the natural steroid hormones that remained in the effluent from the aerobic treatment. A yeast two-hybrid assay was performed to evaluate hormonal degradation. Significant reductions in the concentrations of total VFA, BOD_5, COD_cr, TOC, TS, VSS, and natural steroid hormones were demonstrated in the effluent from the biological treatments. The removal ratios of such concentrations were 99.7%, 90%, 79%, 84%, 51%, 58%, and 99%, respectively. Although the concentrations of the remaining TOC and COD_cr remained constant, natural steroid hormones were completely removed by ozone oxidation.Microbial community in anaerobic digester for synthetic wastewater treatment containing 17β-estradiol was analyzed under the conditions at 8 g/l of TOC loading rate and at 0.05 d-1 of dilution rate. Bacterial-16S rRNA gene clone library analysis showed that the phylum Proteobacteria and Firmicutes were predominated in the reactor.

研究了一种筛选牛粪废物的有效处理工艺，特别是降解天然类固醇激素的工艺。该过程的第一步是高温厌氧消化的抽灌过程。在用自来水稀释四倍后，进行连续进料以用于来自厌氧处理的流出物的好氧处理。然后进行间歇式臭氧氧化，以降解好氧处理后的出水中残留的天然类固醇激素。进行酵母双杂交测定以评价激素降解。经生物处理后的出水中总VFA、BOD_5、COD_（cr）、TOC、TS、VSS和天然甾体激素的浓度均显著降低。这些浓度的去除率分别为99.7%、90%、79%、84%、51%、58%和99%。在TOC负荷率为8 g/l、稀释率为0.05d-1的条件下，对处理17β-雌二醇合成废水的厌氧消化池中的微生物群落进行了分析。细菌16 S rRNA基因克隆文库分析表明，变形菌门和厚壁菌门在反应器中占优势。

项目成果

Degradation and Behavior of Natural Steroid Hormones in Cow Manure Waste during Biological Treatment and Ozone Oxidation

生物处理和臭氧氧化过程中牛粪废弃物中天然类固醇激素的降解和行为

• 发表时间: 2007
• 期刊: Journal of Bioscience and Bioengineering 103 (1)
• 作者: Rahyani;Ermawati;Shigeru;Morimura;Yueqin;Tang;Kai;Liu;Shigeru;Morimura;Kenji;Kida
• 通讯作者: Kida

天然ホルモンの嫌気分解および関与する微生物群集の解析

天然激素的厌氧降解及相关微生物群落分析

• 发表时间: 2007
• 作者: 広池 優明;木田 建次;森村 茂;湯 岳琴
• 通讯作者: 湯 岳琴

Anaerobic degradation of natural hormone and phylogenetic analysis of microbial community

天然激素的厌氧降解及微生物群落系统发育分析

• 发表时间: 2007
• 作者: Masaaki;Hiroike;Kenji;Kida;Shigeru;Morimura;Yueqin;Tang
• 通讯作者: Tang

Degradation and behavior of natural steroid hormones in cow manure waste during biological treatments and ozone oxidation

• DOI: 10.1263/jbb.103.27
• 发表时间: 2007-01-01
• 期刊: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
• 影响因子: 2.8
• 作者: Ermawati, Rahyani;Morimura, Shigeru;Kida, Kenji
• 通讯作者: Kida, Kenji