Evolution of cellular acidic ion accumulation in brown algae and its implication in the phylogeny of Dictyotales

从细胞提取物pH值和主要无机物的离子组成中估计的细胞pH值（AnイイD1+イエD1 NHイD24イイD1+イエD1， KイD1+イエD1， MgイD12+イエD1， CaイD12+，イD1 ClイD1-イD1， BrイD1-イD1， NOイD23イエD2イイD1-イD1，D24 D2 D12-D1）研究了10个品种的离子色谱法（Dictyotales、Desmarestiales、Ectocarpales、Chordariales、Scytosiphonales、Dictyosiphonales、Cutleriales、Sporochnales、Laminariales和Fucales）的Phaeophyceae。在顺序中发现的两种物种，Dictyotales、Dictyopteris sp.和Zonaria stipitata Tanaka等人K. Nozawa，新发现的高酸性物质（细胞内的pH值1.1和1.4），并补充到先前报道的Dictyotteris latiuscula （Okamura）Okamura，D. prolifera（Okamura）Okamura，D. repens（Okamura） Bφrgesen和Spatoglossum J. Tanaka。They all contained high concentrations of SOイD24イD2イD12-イD1 perhaps within the vacuoles。Furthermore、Delamarea attanuata （Kjellman）、Rosenvinge （Dictyosiphonales）和Talassiophyllum clathrus （Gmel.）P.et R.（Laminariales） were shown to contain relatively high concentrations of SOイD24イイD2イD12-イD1 balManagement by relatively high concentrations of CaイD12+イD1。Molecular Phylogenetic Study Using rbcL gene sequences supported the notion that the accumulation of cellular acidic substances occurred indently in Desmarestiales and Dictyotales.与Desmarestiales中的情况相反，高度酸性物质在Dictyotales中没有形成单分子组。

Cellular pH estimated from cell extract pH and the ion compositions of major inorganic ions(AnィイD1+ィエD1 NHィイD24ィエD2ィイD1+ィエD1, KィイD1+ィエD1, MgィイD12+ィエD1, CaィイD12+,ィエD1 ClィイD1-ィエD1, BrィイD1-ィエD1, NOィイD23ィエD2ィイD1-ィエD1, SOィイD24ィエD2ィイD12-ィエD1) were studied by ion chromatography in 60 species of 10 orders (Dictyotales, Desmarestiales, Ectocarpales, Chordariales, Scytosiphonales, Dictyosiphonales, Cutleriales, Sporochnales, Laminariales and Fucales) of Phaeophyceae. Two species in the order Dictyotales, Dictyopteris sp. and Zonaria stipitata Tanaka et K.Nozawa, were newly found to be highly acidic (pH 1.1 and 1.4 within cells), in addition to previously reported dictyotalean species, Dictyopteris latiuscula (Okamura) Okamura, D.prolifera (Okamura) Okamura, D.repens (Okamura) Bφrgesen and Spatoglossum crassum J.Tanaka. They all contained high concentrations of SOィイD24ィエD2ィイD12-ィエD1 perhaps within the vacuoles. Furthermore, Delamarea attanuata (Kjellman) Rosenvinge (Dictyosiphonales) and Talassiophyllum clathrus (Gmel.) P.et R. (Laminariales) were shown to contain relatively high concentrations of SOィイD24ィエD2ィイD12-ィエD1 balanced by relatively high concentrations of CaィイD12+ィエD1. Molecular phylogenetic study using rbcL gene sequences supported the notion that the accumulation of cellular acidic substances occurred independently in Desmarestiales and Dictyotales. In contrast to the case in Desmarestiales, highly acidic species did not form monophyletic group in Dictyotales.