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Evolution of cellular acidic ion accumulation in brown algae and its implication in the phylogeny of Dictyotales

褐藻细胞酸性离子积累的演化及其在网盘目系统发育中的意义

基本信息

批准号：
10836012
负责人：
KAWAI Hiroshi
金额：
$ 2.18万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Cellular pH estimated from cell extract pH and the ion compositions of major inorganic ions(AnィイD1+ィエD1 NHィイD24ィエD2ィイD1+ィエD1, KィイD1+ィエD1, MgィイD12+ィエD1, CaィイD12+,ィエD1 ClィイD1-ィエD1, BrィイD1-ィエD1, NOィイD23ィエD2ィイD1-ィエD1, SOィイD24ィエD2ィイD12-ィエD1) were studied by ion chromatography in 60 species of 10 orders (Dictyotales, Desmarestiales, Ectocarpales, Chordariales, Scytosiphonales, Dictyosiphonales, Cutleriales, Sporochnales, Laminariales and Fucales) of Phaeophyceae. Two species in the order Dictyotales, Dictyopteris sp. and Zonaria stipitata Tanaka et K.Nozawa, were newly found to be highly acidic (pH 1.1 and 1.4 within cells), in addition to previously reported dictyotalean species, Dictyopteris latiuscula (Okamura) Okamura, D.prolifera (Okamura) Okamura, D.repens (Okamura) Bφrgesen and Spatoglossum crassum J.Tanaka. They all contained high concentrations of SOィイD24ィエD2ィイD12-ィエD1 perhaps within the vacuoles. Furthermore, Delamarea attanuata (Kjellman) Rosenvinge (Dictyosiphonales) and Talassiophyllum clathrus (Gmel.) P.et R. (Laminariales) were shown to contain relatively high concentrations of SOィイD24ィエD2ィイD12-ィエD1 balanced by relatively high concentrations of CaィイD12+ィエD1. Molecular phylogenetic study using rbcL gene sequences supported the notion that the accumulation of cellular acidic substances occurred independently in Desmarestiales and Dictyotales. In contrast to the case in Desmarestiales, highly acidic species did not form monophyletic group in Dictyotales.

根据细胞提取物pH和主要无机离子的离子组成估计细胞pH（An NH3 D1+ NH3 D1 NH NH3 D24 NH3 D2 NH3 D1 + NH3 D1，K NH3 D1+ NH3 D1，Mg NH3 D12+ NH3 D1，Ca NH3 D12+，NH3 D1 Cl NH3 D1-NH3 D1，Br NH3 D1-NH3 D1，NO NH3 D23 NH3 D2 NH3 D1-NH3 D1，用离子色谱法研究了褐藻纲10目（网藻目、毛叶藻目、外果皮藻目、弦藻目、胞管藻目、网管藻目、杯藻目、孢子藻目、海带目和墨角藻目）60种褐藻中的硫酸根D24、硫酸根D2、硫酸根D12-硫酸根D1）。在已报道的宽网翅蕨（Dictyopteris latiuscula（Okamura）Okamura）、增殖网翅蕨（D.prolifera（Okamura）Okamura）、匍匐网翅蕨（D.repens（Okamura）Bφrgesen）和粗粒匙舌蕨（Spatoglossum crassum J. Tanaka）之外，新发现两种网翅蕨（Dictyopteris sp.）和具柄带藻（Zonaria stipitata Tanaka et K.Nozawa）为强酸性（细胞内pH值分别为1.1和1.4）。它们都含有高浓度的SO ↓ [2] D ↓ [24] D ↓ [2] D ↓ [12]-D ↓ [1]，可能在液泡中。此外，还对网管藻目（Dictyosiphonales）的细叶苔Delamareaattanuata（Kemman）Rosenvinge和网形Talassiophyllumclathrus（Gmel.）P.et R.（海带目）显示含有相对高浓度的SO_（24）D_（24）D_（22）D_（12）-D_（11）D_（12），由相对高浓度的Ca_（24）D_（12）+D_（11）D_（12）平衡。利用rbcL基因序列进行的分子系统发育研究支持了Desmarestiales和Dictyotales中细胞酸性物质积累独立发生的观点。与Desmarestiales的情况相反，高酸性的物种在网藻目中没有形成单系群。