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Phylogeny and evolution of amitochondriate eukaryotes

线粒体真核生物的系统发育和进化

基本信息

批准号：
10836017
负责人：
YANO Taka-aki
金额：
$ 2.18万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10836017/
关键词：
Early Evolution of Eukaryotes Mitochondria Protists Alignment Molecular phylogeny Ribosomal Proteins Heat Shock Protein 70 Peptide Elongation Factors ヴァリンtRNA合成酵素イソロイシンtRNA合成酵素

项目摘要

In order to elucidate early evolution of eukaryotes, we have been studying phylogenetic relationship aimng protists including arnitochondriate ones with the use of multiple molecular sequence data. In this project, we established the methods to get enough DNA needed for gene analyses of the protists, including Giardia lamblia (Diplomonads), Trichon2onas vaginalis, T. tenax (Trichomonads), Entamoeba histolytica (Entamoebidae), Trypanosoma cruzi (Euglenoids), Biastocystis hominis, Encephalitozoon cuniculi, E.heilein, Glugeaplecogiossi (Microsporidia), and Mastigarnoeba balamuthi. On the basis of the DNA of these protists, we cloned and sequenced the genes encoding isoleucyl- and valyl-tRNA synthetases, ribosomal proteins, 7OkDa heat shock proteins, and peptide elongation factors, the sequences of which had yet been detennined. Phylogenetic analyses including these original data suggested 1) that all the sequences from amitochondriate protists clearly show the sequence feature of typical eukaryotic type, 2) that all of the four amitochondriate protists lineages, Diplomonads, Tricbomonads, Entarnoebidae, and Microsporidia lost their mitochondria secondarily during their evolution, and 3) that B. hominis is involved in Stramenopiles and the closest relative of the Stramenopiles is shred by Alveolata.

为了阐明真核生物的早期进化，我们一直在利用多个分子序列数据研究原生生物（包括节线粒体生物）的系统发育关系。本课题建立了蓝氏贾第鞭毛虫、迷走毛滴虫、tenax（毛滴虫）、溶组织内阿米巴（内阿米巴科）、克氏锥虫（眼虫）、人毕囊原虫、兔脑孢子虫、E.heilein、Glugeaplecogiossi（微孢子虫）和Balamuthi鞭毛虫。根据这些原生生物的DNA，我们克隆了编码异亮氨酰-和缬氨酰-tRNA合成酶、核糖体蛋白、70 kDa热休克蛋白和肽延伸因子的基因，并测定了它们的序列。系统发育分析表明：1）所有的无线粒体原生动物序列都具有典型的真核生物序列特征; 2）双单胞菌纲、毛滴虫纲、内孢子虫科和微孢子虫纲4个无线粒体原生动物谱系在进化过程中都发生了线粒体的继发性丢失; 3）B.人与层孔动物有关，而层孔动物的近亲则被泡孔动物撕碎。