权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Isolation of Ehrlichia canis and its molecular and immunological analyses

犬埃利希体的分离及其分子和免疫学分析

基本信息

批准号：
10839019
负责人：
YAMAMOTO Shizuo
金额：
$ 2.11万
依托单位：
Azabu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10839019/
关键词：
Ehrlichia canis Rhipicepha sanguineus canaine ehrlichiosis IFA

项目摘要

Surveys of antibody to Ehrlichia canis by immunofluorescent antibody (IFA) test and E.canis 16S rDNA by nested polymerase chain reaction (PCR) in household and stray dogs in Japan were performed. In serosurvey for E.canis infection, 17.7% (228) of 1,285 stray dogs in Okinawa Prefecture, 2.8% (11) of 399 stray dogs in Kagoshima Prefecture, 4.9% (22) of 448 private household dogs in Yamaguchi-shi, Yamaguchi Prefecture (6.2% (19) of 303 outdoor dogs were positive and all of 82 indoor dogs were negative.), and 2.3% (8) of 341 household dogs in Tokushima-shi, Tokushima Prefecture (8.3% (3) of 36 dogs infected with babesiosia gibsoni and 1.6% (5) of 305 dogs uninfected babesiosia gibsoni) were showed IFA positive.PCRs for detection of E.canis, E.muris and E.platys 16S rDNA for IFA-positive samples were performed. Three of 70 stray dogs (4.3%) in Okinawa Prefecture and 1 of 11 stray dogs (9.1%) in Kagoshima Prefecture were positive for E.canis PCR.The DNA fragments of E.muris and E.platys were not amplified. The determination of sequences for partial DNA fragments (389-bp) of 16S rDNA in four PCR-positive samples were carried out. All samples were showed quite the same sequences of the partial DNA fragments. This sequence was identical with E.canis isolated in China by Pan et al. in 2000. However, it differed from E.canis Oklahoma strain, in the United State of America, in only one base.

通过免疫荧光抗体（IFA）测试对犬埃里希体抗体进行了调查，并通过巢式聚合酶链反应（PCR）对日本的家犬和流浪狗进行了犬埃里希体抗体16S rDNA调查。在犬艾美耳球虫感染血清调查中，冲绳县1,285只流浪狗中17.7%（228只）、鹿儿岛县399只流浪狗中2.8%（11只）、山口县山口市448只私人家犬中4.9%（22只）（室外狗303只中6.2%（19只）、室内82只狗全部呈阳性）狗是德岛县德岛市的 341 只家犬中有 2.3%（8 只）（感染吉氏巴贝虫的 36 只狗中的 8.3%（3 只）和 305 只未感染吉氏巴贝虫的狗中的 1.6%（5 只）的 IFA 呈阳性。用于检测犬艾美耳球虫、鼠艾美耳球虫和普拉提艾美耳虫 16S rDNA 的 PCR 检测IFA 阳性样本进行了。冲绳县70只流浪狗中的3只（4.3%）和鹿儿岛县11只流浪狗中的1只（9.1%）犬艾美耳球菌PCR呈阳性。鼠艾美耳球菌和鸭艾美耳球虫的DNA片段未扩增。对四个 PCR 阳性样品中 16S rDNA 的部分 DNA 片段（389-bp）进行了序列测定。所有样本均显示出完全相同的部分 DNA 片段序列。该序列与 Pan 等人在中国分离的 E.canis 相同。 2000 年。然而，它与美利坚合众国的 E.canis Oklahoma 菌株仅在一个碱基上有所不同。