The effects of endocrine disrupting chemicals on wildlife

本研究探讨了环境污染物在野生动物和实验动物生殖器官和肝脏中的积累水平及其对激素稳态的影响。我们特别关注了环境污染物对食鱼野生动物的影响，因为这些物种积累了许多亲脂化合物。为了评估接触氯代烃对激素的影响，测定了从日本北海道沿海水域采集的长尾海豹和带状海豹脂肪中的氯代烃（CHCs）水平。在带状密封中，总T3水平随着二邻苯二甲酸多氯联苯（PCB170和180）残留量的增加而显著降低。我们还测定了虎头海雕和白尾海雕胸肌中的有机氯浓度。我们检测到多氯联苯、滴滴涕等有机农药含量较高，可能造成生殖毒性。此外，我们还研究了其他重金属在野生动物体内的积累水平，并检测了高浓度的三丁基锡（TBT）。因此，我们研究了TBT对发育中的实验动物的影响。妊娠大鼠从妊娠第5天（GD 5）至产后第25天（PND 25）给予玉米油或TBT。TBT对幼鼠下丘脑和小脑胶质细胞分化有短暂性抑制作用。

In this study, we investigated the accumulation levels of environmental pollutants and their effects on the hormonal homeostasis in reproductive organs and livers of wild life or experimental animals. Especially, we focused on the effects of environmental pollutants on fish-eating wild life, since numerous lipophilic compounds were accumulated in these species.Chlorinated hydrocarbon (CHCs) levels in the blubber of Larga seals and Ribbon seals collected from the coastal waters of Hokkaido, Japan were determined in order to assess the hormonal effects of CHC exposure. In Ribbon seals, total T3 levels significantly decreased with an increase of di-ortho PCB (PCB170 and 180) residues. We also determined the organochlorine concentrations in the breaset muscle of Steller's sea eagles and white-tail sea eagles. We detected PCB, DDT and other organopesticides at high levels, which was possible to cause reproductive toxicity. Furthermore, we investigated the accumulation levels of other heavy metals and detected tri-butyltin (TBT) in wild life at high concentration. Thus, we investigated the effects of TBT on the developing experimental animals. The pregnant rats were administrated corn oil or TBT from gestation day (GD 5) through postnatal day (PND) 25. The glial cell differentiations in hypothalamus and cerebellum of pups were transiently suppressed by TBT treatment.