New photoswitches for the reversible modulation of DNA and RNA

用于 DNA 和 RNA 可逆调节的新型光电开关

• 批准号: 529549043
• 负责人: Professor Dr. Andres Jäschke
• 金额: --
• 依托单位: Abteilung Pharmazeutische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/529549043?language=en
• 关键词: New; photoswitches; reversible; modulation; DNA

The reversible modulation of DNA and RNA function by light holds great significance for the advancement of bionanotechnology, photopharmacology, super-resolution microscopy, and next-generation computing. Our group has introduced new classes of nucleoside-based diarylethenes (DAEs) and optimized them for high-performance photoswitching. Initial applications at the macromolecular level have been explored. Our long-term objective is to achieve control over the structure and the catalytic, substrate, or regulatory functions of DNA and RNA in a flexible and comprehensive manner. To accomplish this goal, we propose to: i. Generate improved photoswitchable fluorescence with nucleosidic DAEs. ii. Develop dA and dU DAE switches that can be switched with visible light. iii. Optimize dG and dC DAE switches. iv. Create combinatorial methods for determining the optimal positions for photomodulation of deoxyribozymes and DNA aptamers. v. Synthesize and characterize ribo versions of the optimized deoxyribo DAEs. vi. Conduct first proof-of-principle experiments to study DAE-functionalized RNA. vii. Devise combinatorial methods for identifying the optimal positions for photomodulation of ribozymes and RNA aptamers. This work will enable applications in the fields mentioned above.

光对DNA和RNA功能的可逆调节对生物纳米技术、光药理学、超分辨率显微镜和下一代计算机技术的发展具有重要意义。我们的团队已经引入了新型的核苷基二乙烯（DAEs），并对它们进行了优化，以实现高性能的光电开关。在大分子水平上的初步应用已经进行了探索。我们的长期目标是以灵活和全面的方式实现对DNA和RNA的结构和催化，底物或调节功能的控制。为了实现这一目标，我们提出：i.利用核苷DAEs产生改进的光开关荧光。2。开发可以用可见光切换的dA和dU DAE开关。3。优化dG和dC DAE开关。iv.创造组合方法来确定脱氧核酶和DNA适体的最佳光调节位置。v.合成并表征优化的脱氧核糖DAEs的核糖版本。vi.进行第一次原理验证实验，研究大功能RNA。7。设计组合方法以确定核酶和RNA适体的最佳光调节位置。这项工作将使上述领域的应用成为可能。