New photoswitches for the reversible modulation of DNA and RNA

光对DNA和RNA功能的可逆调节对于生物纳米技术、生物药理学、超分辨率显微镜和下一代计算的进步具有重要意义。我们的团队已经引入了新的基于核苷的二芳基乙烯（DAE）类，并优化了它们的高性能光开关。在大分子水平的初步应用进行了探索。我们的长期目标是以灵活和全面的方式实现对DNA和RNA的结构和催化，底物或调节功能的控制。为实现这一目标，我们建议：使用核苷DAE产生改进的光可切换荧光。二.开发可通过可见光进行切换的dA和dU DAE交换机。三.优化dG和dC DAE交换机。四.创建组合方法，用于确定脱氧核酶和DNA适体光调制的最佳位置。v.合成和表征优化的脱氧核糖DAE的核糖形式。六.进行第一次原理验证实验，以研究DAE功能化的RNA。七.设计组合方法来确定核酶和RNA适体光调节的最佳位置。这项工作将使上述领域的应用。

The reversible modulation of DNA and RNA function by light holds great significance for the advancement of bionanotechnology, photopharmacology, super-resolution microscopy, and next-generation computing. Our group has introduced new classes of nucleoside-based diarylethenes (DAEs) and optimized them for high-performance photoswitching. Initial applications at the macromolecular level have been explored. Our long-term objective is to achieve control over the structure and the catalytic, substrate, or regulatory functions of DNA and RNA in a flexible and comprehensive manner. To accomplish this goal, we propose to: i. Generate improved photoswitchable fluorescence with nucleosidic DAEs. ii. Develop dA and dU DAE switches that can be switched with visible light. iii. Optimize dG and dC DAE switches. iv. Create combinatorial methods for determining the optimal positions for photomodulation of deoxyribozymes and DNA aptamers. v. Synthesize and characterize ribo versions of the optimized deoxyribo DAEs. vi. Conduct first proof-of-principle experiments to study DAE-functionalized RNA. vii. Devise combinatorial methods for identifying the optimal positions for photomodulation of ribozymes and RNA aptamers. This work will enable applications in the fields mentioned above.