RESEARCH ON THE INTER-PLANTS COMMUNICATION MECHANISM FOR CRISIS SIGNAL UPON INSECT ATTACK BY MEANS OF GENETIC mRNA ANALYSIS AND NON-DESTRUCTIVE OPTO-ELECTRO-PHYSIOLOGICAL MEASUREMENTS OF SELF-HEALING OR PROTECTING BEHAVIORS AGAINST THE INJURY.

在受昆虫侵害的植物中检测到mRNA的遗传表达，其指示用于自我修复和保护免受损伤的分泌物的产生。同样的mRNA在另一种植物中检测到，这是没有受到昆虫的损害，只是在受害植物周围。为了揭示植物间的信息传递机制和途径，我们开发了几种检测分析系统，即遗传分析系统、生物光子成像与计数系统、叶绿素荧光检测系统、生物电位信号声发射信号检测系统、光合成/蒸腾测定系统和一个带有气密隔离百叶窗的屏蔽舱，将舱内分为两个房间，每个房间分别放置受伤和未受伤的植物。对同一叶片的生物电位信号、叶绿素荧光和光合响应有显著影响。当其中一片叶片受到相同的热（50℃）或冷（5℃）刺激时，在20℃无刺激条件下，其它叶片也有类似的反应，这表明对某一片叶片的热或冷刺激能传递到另一片叶片。水稻受害叶片与未受害叶片的mRNA检测结果存在显著差异。结果表明，遗传分析技术与其它无损、真实的实时光电测量技术相结合的方法，对于研究植物间的通讯机制或途径是可行和有效的。

Genetic expression of mRNA, which indicates generation of the secretion for self-healing and protection against the injury, is detected in a plant inflicted by insects. The same mRNA is detected in the other plant, which is not damaged by the insects and is just around the injure plant. This suggests that communication mechanism or route exists for transmitting the injury crisis signal to other plants.To reveal the inter-plant communication mechanism or routes, we have developed several measuring and analysis systems, that is, genetic analysis system, bio-photon imaging and counting system, chlorophyll fluorescence measuring system , bio-electric potential signal acoustic emission signal measuring system, photo-sythesis/transpiration measuring system and a shielded cabin with air-tight partition shutter, dividing the cabin into two rooms , each for the plant with injury and without injury repectively.When pin-point hot (50℃) or cold (5℃) stimuli is given on a leaf of Kapok (Schefflera arboricola). bio-electric potential signal, chlorophyll fluorescence and photosynthetic response on the same leaf is affected remarkably. When the same pin-point hot (50℃) or cold (5℃) stimuli is given on one of the leaves, quite similar responses mentioned above are observed on the other leaves at 20℃ without stimuli.This suggests that hot or cold stimuli given on certain leaf is transmitted to the other leave. Siginificant difference has been confirmed in the data between those of mRNA detected for the leaves of rice plant injured by insects and those without injury. The same difference has been observed between the data for bio-photon on the leaves with and without injury.Availablity and effectiveness of the method of integrating the genetic analysis scheme and other non-destructive, real time opto-elctronic measuring skills has been proved for the study of the inter-plant communication mechanisms or routes.