RESEARCH ON THE INTER-PLANTS COMMUNICATION MECHANISM FOR CRISIS SIGNAL UPON INSECT ATTACK BY MEANS OF GENETIC mRNA ANALYSIS AND NON-DESTRUCTIVE OPTO-ELECTRO-PHYSIOLOGICAL MEASUREMENTS OF SELF-HEALING OR PROTECTING BEHAVIORS AGAINST THE INJURY.

在受昆虫侵害的植物中检测到mRNA的遗传表达，这表明产生了自我修复和保护免受伤害的分泌。同样的mRNA在另一株植物中被检测到，它没有被昆虫破坏，只是在受伤的植物周围。这表明植物间存在着将伤害危机信号传递给其他植物的沟通机制或途径。为了揭示植物间的通讯机制或途径，我们开发了几种测量和分析系统，即遗传分析系统、生物光子成像和计数系统、叶绿素荧光测量系统、生物电势信号声发射信号测量系统、光合/蒸腾测量系统和带密闭隔断百叶窗的屏蔽舱，将舱室分为两个房间。分别对有伤株和无伤株进行分析。当针尖热（50℃）或冷（5℃）刺激木棉叶子（Schefflera arboricola）。同一叶片上的生物电位信号、叶绿素荧光和光合响应受到显著影响。当对其中一片叶片施加相同的针尖热（50℃）或冷（5℃）刺激时，在没有刺激的20℃条件下，其他叶片也观察到类似的反应。这表明某片叶子上的热或冷刺激会传递到另一片叶子上。受虫害水稻叶片mRNA的检测结果与未受虫害水稻叶片mRNA的检测结果存在显著差异。在有无损伤的叶片上观察到相同的差异。将遗传分析方案与其他无损、实时光电测量技术相结合的方法在研究植物间通讯机制或途径方面的有效性和有效性已得到证实。

Genetic expression of mRNA, which indicates generation of the secretion for self-healing and protection against the injury, is detected in a plant inflicted by insects. The same mRNA is detected in the other plant, which is not damaged by the insects and is just around the injure plant. This suggests that communication mechanism or route exists for transmitting the injury crisis signal to other plants.To reveal the inter-plant communication mechanism or routes, we have developed several measuring and analysis systems, that is, genetic analysis system, bio-photon imaging and counting system, chlorophyll fluorescence measuring system , bio-electric potential signal acoustic emission signal measuring system, photo-sythesis/transpiration measuring system and a shielded cabin with air-tight partition shutter, dividing the cabin into two rooms , each for the plant with injury and without injury repectively.When pin-point hot (50℃) or cold (5℃) stimuli is given on a leaf of Kapok (Schefflera arboricola). bio-electric potential signal, chlorophyll fluorescence and photosynthetic response on the same leaf is affected remarkably. When the same pin-point hot (50℃) or cold (5℃) stimuli is given on one of the leaves, quite similar responses mentioned above are observed on the other leaves at 20℃ without stimuli.This suggests that hot or cold stimuli given on certain leaf is transmitted to the other leave. Siginificant difference has been confirmed in the data between those of mRNA detected for the leaves of rice plant injured by insects and those without injury. The same difference has been observed between the data for bio-photon on the leaves with and without injury.Availablity and effectiveness of the method of integrating the genetic analysis scheme and other non-destructive, real time opto-elctronic measuring skills has been proved for the study of the inter-plant communication mechanisms or routes.