Development of Forced Growth System for Antioxidant AHCC(Agaricus, Glucans)-enriched Medical Mushroom on the basis of Speaking Plant Approach utilizing Bio-electronic sensing and Optical-CT Imaging

抗氧化剂AHCC，如蘑菇或葡聚糖，被称为抗癌化合物。在所谓的药用蘑菇中发现了少量的这些化合物。从富含AHC的蘑菇中有效地提取这些化合物将为药用蘑菇的养殖提供基础。利用在蘑菇上观察到的生物电势信号来检测AHC的生成和蘑菇生长的活性，考察了不同养殖条件下AHC的最适条件。此外，还生产了一套精确的生物电势信号自动测量系统。利用制作的设备所获得的知识，制作了一个自动系统，用于测量由可编程空气成分或光刺激产生的生物电势。以蘑菇本身为生物传感器，针对最佳栽培条件，尝试了光源设备的实际应用。利用磁共振成像，我们成功地产生了从真菌最初种植到子实体孢子散布的一段时间的真菌断层图像的无损显示。根据在实验室培养箱中获得的蘑菇培养结果，为了评价该系统的有效性，观察了特定蘑菇的生物节律，利用它作为生物传感器来控制光照和黑暗对周围蘑菇的刺激时长，以证实所谓的“说话植物法”用于培养药用菌生产抗癌化合物的可行性。

Antioxidant AHCC such as Agaricus or glucans has been known as anticancer compound.Small amount of these compounds are found in so-called medical mushrooms.Effective extraction of these compound from the AHCC enriched mushrooms will promise a mushroom farming for medical use.Feasibility of the detection of AHCC enriched points in medical mushrooms have been investigated by a newly developed 2 dimensional Optical CT. The optimum condition has been surveyed toward higher enrichment for AHCC over various farming conditions utilizing bioelectric potential signals observed on the mushroom to sense the activity of generating AHCC and mushroom growth.Additional production of a precise auto-measurement system for bioelectric potential signals. Using the knowledge gained from the fabricated equipment, an automatic system was produced for the measurement of the bioelectric potential, resulting from programmable air components or light stimuli.Practical use of light source equipment has been tried aiming at the optimum farming conditions, using the mushroom itself as a biosensor.Using MRI, we have succeeded in generating a non-destructive display of a tomograph of fungus for a period covering the initial planting of the fungus up to the dispersal of spores from the fruiting body.A light source system was fabricated, based on the results obtained from mushroom farming in a Growth Chamber in the laboratory in order to evaluate the effectiveness of the system.Observation of the biorhythm of a specific mushroom, using it as a biosensor to control the duration of light and dark of light stimulation of the surrounding mushrooms to confirm the feasibility of so-called "Speaking Plant Approach for the farming of medical mushrooms to produce anti-cancer compounds.