Development of Forced Growth System for Antioxidant AHCC(Agaricus, Glucans)-enriched Medical Mushroom on the basis of Speaking Plant Approach utilizing Bio-electronic sensing and Optical-CT Imaging

抗氧化剂AHCC（Agaricus）或葡聚糖是一种抗癌物质，在药用蘑菇中也有少量的AHCC。从富含AHCC的蘑菇中有效提取这些化合物，将为药用蘑菇的种植提供希望。利用新开发的二维光学CT技术，研究了检测药用蘑菇中AHCC富集点的可行性。利用蘑菇上的生物电位信号来检测AHCC的产生和蘑菇生长的活性，探索了在不同栽培条件下AHCC富集的最佳条件，并建立了一套高精度的生物电位信号自动检测系统。利用从制造的设备中获得的知识，生产了用于测量生物电势的自动系统，所述生物电势由可编程空气成分或光刺激产生。针对最佳养殖条件，使用蘑菇本身作为生物传感器，尝试了光源设备的实际使用。使用MRI，我们已经成功地在覆盖真菌的最初种植直到孢子从子实体散布的时期内产生真菌的断层图像的非破坏性显示。制造了光源系统，基于在实验室的生长室中从蘑菇养殖获得的结果，以评估该系统的有效性。观察特定蘑菇的生物节律，利用它作为生物传感器来控制周围蘑菇的光刺激的光和暗的持续时间，以确认所谓的“说话植物方法"用于药用蘑菇的养殖以产生抗致癌化合物

Antioxidant AHCC such as Agaricus or glucans has been known as anticancer compound.Small amount of these compounds are found in so-called medical mushrooms.Effective extraction of these compound from the AHCC enriched mushrooms will promise a mushroom farming for medical use.Feasibility of the detection of AHCC enriched points in medical mushrooms have been investigated by a newly developed 2 dimensional Optical CT. The optimum condition has been surveyed toward higher enrichment for AHCC over various farming conditions utilizing bioelectric potential signals observed on the mushroom to sense the activity of generating AHCC and mushroom growth.Additional production of a precise auto-measurement system for bioelectric potential signals. Using the knowledge gained from the fabricated equipment, an automatic system was produced for the measurement of the bioelectric potential, resulting from programmable air components or light stimuli.Practical use of light source equipment has been tried aiming at the optimum farming conditions, using the mushroom itself as a biosensor.Using MRI, we have succeeded in generating a non-destructive display of a tomograph of fungus for a period covering the initial planting of the fungus up to the dispersal of spores from the fruiting body.A light source system was fabricated, based on the results obtained from mushroom farming in a Growth Chamber in the laboratory in order to evaluate the effectiveness of the system.Observation of the biorhythm of a specific mushroom, using it as a biosensor to control the duration of light and dark of light stimulation of the surrounding mushrooms to confirm the feasibility of so-called "Speaking Plant Approach for the farming of medical mushrooms to produce anti-cancer compounds.