Multi-functional biosensing chip for rapid determination of herbicides for paddy fields

快速测定稻田除草剂的多功能生物传感芯片

基本信息

批准号：
11650832
负责人：
SUZUKI Masayasu
金额：
$ 2.24万
依托单位：
Toyama University (2000)Kyushu Institute of Technology (1999)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650832/
关键词：
bio-sensing eco-toxicity herbicide chronic toxicity acute toxicity green algae immobilization chlorophyll

项目摘要

This study aims at the basic research for the future development of the integrated biosensing chip for evaluation of acute ecotoxicity indices (inhibition of fluorescence or oxygen evolution) and chronic ecotoxicity index (growth inhibition). In this study, we have investigated the following points : 1)Survey of immobilization method for green algae, 2) Chronic ecotoxicity test by using immobilized green algae, 3) Freezing method of green algae, 4) Quantitative evaluation of inhibitions of fluorescence and oxygen evolution in green algae caused by herbicides, 5) acute toxicity test by using immobilized green algae.As a green algae immobilization material, photo-crosslinkable prepolymer PVA-SbQ was most suitable. Immobilized green algae showed the similar growth rate to free green algae. By chronic ecotoxicity test using immobilized green algae, most of the herbicides could be detected.The green algae frozen with 5% dimethyl sulfoxide showed good viability and growth rate after thawing out. Chronic ecotoxicity test also could be done by using this green algae. By the combination of this freezing technique and micro plate assay method, a quite simple novel ecotoxicity test method could be developed.The inhibition of photosynthetic activity could change chlorophyll-fluorescence intensity and oxygen evolution rate. Ecotoxicity of herbicides was evaluated with fluorescence intensity change and oxygen concentration change. Triazine group herbicides could be successfully determined by this method within an hour.Finally, a green algae immobilized membrane was set at the sensing area of an oxygen electrode, and atrazine and CAT were determined by using this algae-electrode.

这项研究旨在为综合生物传感芯片的未来发展提供基础研究，以评估急性生态毒性指数（抑制荧光或氧气进化）和慢性生态毒性指数（生长抑制）。在这项研究中，我们研究了以下几点：1）使用固定的绿色藻类对绿藻的固定方法的调查，2）慢性生态毒性测试，3）绿色藻类的冻结方法，4）绿色藻类对绿色藻类在绿色藻类中的抑制作用的定量评估，使用绿色藻类在绿色藻类中抑制绿色藻类，使用急性毒性，5）急性毒性，5）急性毒性，5）藻类固定材料，可连接的前聚合物PVA-SBQ是最合适的。固定的绿藻显示出与游离绿藻相似的生长速率。通过使用固定绿藻类的慢性生态毒性测试，可以检测到大多数除草剂。绿藻冻结了5％二甲基亚氧化藻，解冻后显示出良好的活力和生长速率。慢性生态毒性测试也可以使用这种绿藻进行。通过这种冰点技术和微板测定法的结合，可以开发出一种非常简单的新型生态毒性测试方法。光合作用活性的抑制可能会改变叶绿素荧光强度和氧气的演化速率。用荧光强度变化和氧气浓度变化评估除草剂的生态毒性。三嗪组除草剂可以在一个小时内通过该方法成功确定。在最后，在氧电极的传感区域设置了固定的绿藻类，并使用这些藻类电极确定了阿特拉津和CAT。