レーザアブレーションが引き起こす生体系組織コラーゲン膜の光-熱変換機

生物系统中组织胶原膜中激光烧蚀诱导的光热转换器

• 批准号: 11650850
• 负责人: ISHII Tadahiro
• 金额: $ 2.24万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650850/
• 关键词: Collagen; Laser Ablation; Time-resolved measurements; Excimer Laser; Irradiation Conditions; Denaturation; Alignment; Surface Microstructure

In order to gain obvious insight into the role of water in the mechanism of the excimer laser ablation of the cornea, we have macroscopically investigated the ablation behavior of collagen gel in swelled states by direct photoetching using an ArF excimer laser with time-resolved photography, and furthermore the thermal effects on the microscopic structures of collagen -olecules by FTIR-ATR spectroscopy. As for the dried collagen film (DF), a well-defined periodic structure ascribed to a Fresnel diffraction of the laser beam at the edge of the aperture was observed in the irradiated region by AFM, whereas for the hydrated collagen film (HF), the irregularity of the surface structure for HF was created by the heat through photo-thermal conversion and/or boiling of water in the gel matrix. FTIR-ATR spectroscopic results revealed that the existence of the water suppressed the denaturation of collagen to gelatin on the surface in the irradiated region. After all, it was inferred that during the ablation process for HF,the laser energy would be consumed as the latent heat of evaporation of water, accompanied by the suppression of the increment in the surface temperature in the irradiated region and further for the denaturation of the collagen.

为了使水的作用明显了解角膜的含量激光消融机制，我们通过使用时间分辨的摄影和胶合物对胶原蛋白效果直接使用ARF Excimer激光直接对胶原蛋白激光直接进行胶原蛋白激光，从而在膨胀状态下进行宏观研究。光谱法。至于干燥的胶原蛋白膜（DF），AFM在辐射的区域中观察到了归因于光圈边缘的激光束衍射的明确定义的周期性结构，而水合胶原蛋白膜（HF）的辐射区域，而HF的表面结构的不规则结构是由热量通过摄影而造成的。 FTIR-ATR光谱结果表明，水的存在抑制了胶原蛋白对辐照区域表面上的明胶变性。毕竟，可以推断在HF的消融过程中，激光能量将作为水的潜热热量消耗，并伴随着辐照区域中表面温度的增量抑制，进一步抑制了胶原蛋白的变性。

项目成果

Masaru Sugiura, Katsunori Tsunoda, Hirofumi Yajima, Tadahiro Ishii, Hiroshi Ito, and Yutaka Nagai: "Submicroscopic Morphology of the Laser Induced Surface Structure of Partially Renatured Gelatin Films"Chemistry Letters. 2001. (2001)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Ito 和 Yutaka Nagai：“激光诱导部分复性明胶薄膜表面结构的亚显微形态”化学快报。

Masaru Sugiura,Katsunori Tsunoda,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Submicroscopic Morphological Changes of Laser Induced Surface Structure of Collagen, Tendon, and Gelatin Films"Chemistry Letters. 1288-1289 (2000)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“激光诱导胶原蛋白、肌腱和明胶膜表面结构的亚显微形态变化”化学快报。

Masaru Sugiura,Katsunori Tsunoda,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Submicroscopic Morphology of the Laser Induced Surface Structure of Partially Renatured Gelatin Films"Chemistry Letters. (2001)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“激光诱导部分复性明胶薄膜表面结构的亚显微形态”化学快报。

Masaru Sugiura,Katsunori Tsunoda,Masashi Sonoyama,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Characterization of Water Contribution to Excimer Laser Ablation of Collagen"Journal of Photochemistry and Photobiology A : Chemistry. (2001)

Masaru Sugiura、Katsunori Tsunoda、Masashi Sonoyama、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“水对胶原蛋白准分子激光消融作用的表征”光化学和光生物学杂志 A：化学。

Katsunori Tsunoda, Masaru Sugiura, Masashi Sonoyama, Hirofumi Yajima, Tadahiro Ishii, Hiroshi Ito, and Yutaka Nagai: "Characterization of Water Contribution to Excimer Laser Ablation of Collagen"Journal of Photochemistry and Photobiology A Chemistry. (200

Katsunori Tsunoda、Masaru Sugiura、Masashi Sonoyama、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Ito 和 Yutaka Nagai：“水对胶原蛋白准分子激光消融作用的表征”光化学和光生物学 A 化学杂志。