レーザアブレーションが引き起こす生体系組織コラーゲン膜の光-熱変換機

生物系统中组织胶原膜中激光烧蚀诱导的光热转换器

• 批准号: 11650850
• 负责人: ISHII Tadahiro
• 金额: $ 2.24万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650850/
• 关键词: Collagen; Laser Ablation; Time-resolved measurements; Excimer Laser; Irradiation Conditions; Denaturation; Alignment; Surface Microstructure

In order to gain obvious insight into the role of water in the mechanism of the excimer laser ablation of the cornea, we have macroscopically investigated the ablation behavior of collagen gel in swelled states by direct photoetching using an ArF excimer laser with time-resolved photography, and furthermore the thermal effects on the microscopic structures of collagen -olecules by FTIR-ATR spectroscopy. As for the dried collagen film (DF), a well-defined periodic structure ascribed to a Fresnel diffraction of the laser beam at the edge of the aperture was observed in the irradiated region by AFM, whereas for the hydrated collagen film (HF), the irregularity of the surface structure for HF was created by the heat through photo-thermal conversion and/or boiling of water in the gel matrix. FTIR-ATR spectroscopic results revealed that the existence of the water suppressed the denaturation of collagen to gelatin on the surface in the irradiated region. After all, it was inferred that during the ablation process for HF,the laser energy would be consumed as the latent heat of evaporation of water, accompanied by the suppression of the increment in the surface temperature in the irradiated region and further for the denaturation of the collagen.

为了更好地了解水在准分子激光角膜消融机理中的作用，我们用ArF准分子激光直接光刻法和时间分辨照相法对溶胀状态下胶原凝胶的消融行为进行了宏观研究，并进一步用FTIR-ATR光谱法研究了热效应对胶原分子微观结构的影响。对于干燥的胶原蛋白膜（DF），一个定义明确的周期性结构归因于菲涅耳衍射的激光束在孔径的边缘观察到在照射区域通过AFM，而对于水合的胶原蛋白膜（HF），HF的表面结构的不规则性是由热通过光热转换和/或沸腾的水在凝胶基质中。FTIR-ATR光谱结果表明，水的存在抑制了辐照区域表面胶原蛋白向明胶的变性。毕竟，据推断，在HF的烧蚀过程中，激光能量将作为水的蒸发潜热消耗，伴随着照射区域的表面温度的增量的抑制，并进一步用于胶原的变性。

项目成果

Masaru Sugiura, Katsunori Tsunoda, Hirofumi Yajima, Tadahiro Ishii, Hiroshi Ito, and Yutaka Nagai: "Submicroscopic Morphology of the Laser Induced Surface Structure of Partially Renatured Gelatin Films"Chemistry Letters. 2001. (2001)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Ito 和 Yutaka Nagai：“激光诱导部分复性明胶薄膜表面结构的亚显微形态”化学快报。

Masaru Sugiura,Katsunori Tsunoda,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Submicroscopic Morphological Changes of Laser Induced Surface Structure of Collagen, Tendon, and Gelatin Films"Chemistry Letters. 1288-1289 (2000)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“激光诱导胶原蛋白、肌腱和明胶膜表面结构的亚显微形态变化”化学快报。

Masaru Sugiura,Katsunori Tsunoda,Masashi Sonoyama,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Characterization of Water Contribution to Excimer Laser Ablation of Collagen"Journal of Photochemistry and Photobiology A : Chemistry. (2001)

Masaru Sugiura、Katsunori Tsunoda、Masashi Sonoyama、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“水对胶原蛋白准分子激光消融作用的表征”光化学和光生物学杂志 A：化学。

Masaru Sugiura,Katsunori Tsunoda,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Submicroscopic Morphology of the Laser Induced Surface Structure of Partially Renatured Gelatin Films"Chemistry Letters. (2001)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“激光诱导部分复性明胶薄膜表面结构的亚显微形态”化学快报。

Masaru Sugiura,Katsunori Tsunoda,Hirofumi Yajima,Tadahiro Ishii,Hiroshi Itoh,and Yutaka Nagai: "Morphology of the Laser Induced Surface Structure of Partially Renatured Gelatin Films"Chemistry Letters. (2001)

Masaru Sugiura、Katsunori Tsunoda、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Itoh 和 Yutaka Nagai：“激光诱导部分复性明胶薄膜表面结构的形态”化学快报。