Breeding study of new variety groups in the genus Rhododendron based on molecular phylogenic analysis

基于分子系统发育分析的杜鹃属新品种群选育研究

• 批准号: 11660021
• 负责人: HANDA Takashi
• 金额: $ 2.3万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660021/
• 关键词: Rhododendron; Menziesia; Molecular Phytogeny; Interpeneric Hybrid; Amplified Fragment Length Polymorphisms; ITS; ツツジ属; 遠縁交雑; matK

To develop new cultivar groups in the genus Rhododendron, sequence analysis and AFLP analysis were performed. As a result of matK region of CPDNA and ITS region of nrDNA in Rhododendron and its close related species, taxonomic position of some species should be reconstituted in the genus Rhododendron. All five species of the genus Menziesia were included in the clade Rhododendoron, which indicates Menziesia might be a submenus of Rhododendron. AFLP analysis could reveal the genetic diversity of Menziesia.Some combinations for cross-hybridization between M multittora and several species of Rhododendron produced seedlings. These seedlings were analyzed for AFLP analysis to confirm the hybridity. Microscopic observation of elongation of pollen tube and embryo development were performed to clarify the reason of difficulty in some combinations.

为开发杜鹃花属新品种群，对杜鹃花属植物进行了序列分析和AFLP分析。杜鹃花属及其近缘种的CPDNA的matK区和nrDNA的ITS区的存在，使一些种在杜鹃花属中的分类地位有可能被重新确定。这5种植物均被纳入杜鹃花属的分支中，表明它们可能是杜鹃花属的一个亚目。AFLP分析可以揭示蒙紫杜鹃的遗传多样性，蒙紫杜鹃与几种杜鹃属植物的杂交组合产生了幼苗。对这些幼苗进行AFLP分析以确认杂交性。通过对花粉管伸长和胚发育过程的显微观察，阐明了某些组合出现困难的原因。

项目成果

Kurashige, Y., J-I. Etoh, T. Handa, K. Takayanagi, T. Yukawa: "Sectional relationships in the genus Rhododendron (Ericaceae) : evidence from matK and trnK intron sequences"Plant Systematics and Evolution. 228. 1-14 (2001)

仓重，Y.，J-I。

Y.Kurashige, J-1 Etoh, T.Handa, K.Takayanagi, T.Yukawa: "Sectional relathionships in the genes Rhodudendron (Ericaceae) : evidence from matk and trnk intror sequences"Plant Systematics and Evolution. 228. 1-14 (2001)

Y.Kurashige、J-1 Etoh、T.Handa、K.Takayanagi、T.Yukawa：“杜鹃花（杜鹃花科）基因中的部分关系：来自 matk 和 trnk 内含序列的证据”植物系统学和进化。

Y.Kurashige, J.-I.Etoh, T.Handa, K.Takayanagi, T.Yukawa: "Sectional relationships in the genus Rhododendron (Ericaceae): evidence from matK and trnK intron sequences"Plant Systematics and Evolution. 228. 1-14 (2001)

Y.Kurashige、J.-I.Etoh、T.Handa、K.Takayanagi、T.Yukawa：“杜鹃花属（杜鹃花科）的部分关系：来自 matK 和 trnK 内含子序列的证据”植物系统学和进化。