Breeding study of new variety groups in the genus Rhododendron based on molecular phylogenic analysis

为开发杜鹃属新品种群，进行了序列分析和AFLP分析。由于杜鹃属及其近缘种中cpDNA的matK区和nrDNA的matK区，一些种的分类地位需要重新确定。该属的5种植物均包括在杜鹃花枝中，表明该属可能是杜鹃花属的一个亚菜单。AFLP分析可以揭示多花杜鹃的遗传多样性。多花杜鹃与几种杜鹃杂交的一些组合产生了杜鹃幼苗。对这些幼苗进行AFLP分析，以确认其杂交性。对部分组合的花粉管伸长和胚胎发育进行了显微观察，以明确组合困难的原因。

To develop new cultivar groups in the genus Rhododendron, sequence analysis and AFLP analysis were performed. As a result of matK region of CPDNA and ITS region of nrDNA in Rhododendron and its close related species, taxonomic position of some species should be reconstituted in the genus Rhododendron. All five species of the genus Menziesia were included in the clade Rhododendoron, which indicates Menziesia might be a submenus of Rhododendron. AFLP analysis could reveal the genetic diversity of Menziesia.Some combinations for cross-hybridization between M multittora and several species of Rhododendron produced seedlings. These seedlings were analyzed for AFLP analysis to confirm the hybridity. Microscopic observation of elongation of pollen tube and embryo development were performed to clarify the reason of difficulty in some combinations.