New sex pheromones in marine algae and their biosynthesis.

到目前为止，已经在海洋褐藻中鉴定出11种性信息素，而绿色海藻中的性信息素至今还不清楚。因此，对绿藻信息素进行了探索。1)千叶县千叶县基米加哈马海岸采集的大麦雌、雄配子体的成熟。1月，在14.5℃的空气鼓泡条件下培养3天。雄配子体的吸引证实了雌配子体的成熟。2)成熟配子体的雌配子除了有一滴雄配子悬浮液外，还出现了雄配子的光晕。3)将具有引诱雄性猎物活性的雌性培养液分离为水层和有机层。水溶性组分表现出显著的雄配子吸引活性，在水中仅表现出较弱的活性，并具有褐藻信息素，如麦角蕨烯B(Hormosiene)、麦角蕨烯C‘(Dtyopterene C’)和麦角蕨烯D‘(Dtyopterene D’)。

Up to date, eleven sex pheromones have been identified in marine brown algae, however, the sex pheromones of green marine algae have not been known so far. Thus, the green algal pheromones were explored. 1) The maturation of female and male gametophytes of Bryopsis maxima collected along Kimigahama coast, Chyoshi, Chiba pref. January, was induced under culture for 3 days at 14.5℃ with air bubbling. The maturation of female gametophytes was comfirmed by attraction of male gametes. 2) Female gametes from the matured gametophytes showed a halo of male gametes in addition of a drop of male gamete suspension. 3) The female culture solution having male game-attracting activity was fractionated to a water layer and an organic layer. The water soluble fraction showed a prominent male gamete-attracting activity and only a weak activity in the water in soluble fraction as well as brown algal pheromones such as dictyopterene B (hormosirene), dictyopterene C' (dictyotene) and dictyopterene D' (ectocarpene).