Neuroendocrine regulation of expression of apolipoprotein A-IV gene in intestinal epithelial cells

肠上皮细胞载脂蛋白A-IV基因表达的神经内分泌调控

• 批准号: 11660118
• 负责人: SONOYAMA Kei
• 金额: $ 2.5万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660118/
• 关键词: apolipoprotein AIV; small intestine; Caco-2; gene expression; gut hormone; neural peptide; RT-PCR; 神経系; ヘキサメトニウム; アトロピン; プロプラノロール; ラット

The present study investigated the role of neural and endocrine factors in the regulation of expression of apolipoprotein A-IV (A-IV) gene in intestinal epithelial cells. Intravenous infusion of ganglionic blocker, cholinergic antagonist, and NOS inhibitor suppressed the expression of A-IV mRNA in rat ileum, suggesting regulation of A-IV gene by cholinergic and NOergic neurons. A-TV mRNA was not affected byβ-adrenergic, dopaminergic, serotonergic, ATRergic antagonists. Since ganglionic blocker did not suppress the increase in A-IV mRNA level by dietary lipid and in adaptive ileum after massive small bowel resection, the upregulation is unlikely to be associated with nerve. In Caco-2 intestinal cells, PYY upregulated the A-IV gene in time-and dose-dependent manner. VIP, bombesin, and substance P also increased the A-IV mRNA, while somatostatin28 suppressed it and, adrenaline, carbachol, glucagon, GRP, NPY, CGRP, GLP, and Met-enkephaline had no effect. These results suggest that expression of A-IV gene in intestinal epithelial cells is regulated by a number of factors associated with gut hormones and neural peptides.

本研究探讨了神经和内分泌因素在肠上皮细胞载脂蛋白 A-IV (A-IV) 基因表达调节中的作用。静脉输注神经节阻滞剂、胆碱能拮抗剂和 NOS 抑制剂可抑制大鼠回肠中 A-IV mRNA 的表达，表明胆碱能和 NOergic 神经元对 A-IV 基因的调节。 A-TV mRNA 不受β-肾上腺素能、多巴胺能、5-羟色胺能、ATR 能拮抗剂的影响。由于神经节阻滞剂不能抑制饮食脂质和大面积小肠切除后适应性回肠中 A-IV mRNA 水平的增加，因此上调不太可能与神经有关。在 Caco-2 肠细胞中​​，PYY 以时间和剂量依赖性方式上调 A-IV 基因。 VIP、铃蟾肽和 P 物质也会增加 A-IV mRNA，而生长抑素 28 会抑制它，而肾上腺素、卡巴胆碱、胰高血糖素、GRP、NPY、CGRP、GLP 和 Met-脑啡肽则没有影响。这些结果表明肠上皮细胞中A-IV基因的表达受到与肠道激素和神经肽相关的许多因素的调节。

项目成果

Sonoyama, K.: "Non-effect of hexamethonium, a ganglionic blocker, on the response of ileal apolipoprotein A-IV mRNA following a massive small bowel resection in rats"Biosci.Biotechnol.Biochem.. 64. 1282-1284 (2000)

Sonoyama, K.：“六甲铵（一种神经节阻滞剂）对大鼠大面积小肠切除术后回肠载脂蛋白 A-IV mRNA 的反应无影响”Biosci.Biotechnol.Biochem.. 64. 1282-1284 (2000)

Sonoyama,K.: "Peptide YY stimulates the expression of apolipoprotein A-IV gene in Caco-2 intestinal cells"Proc.Soc.Exp.Biol.Med.. 223. 270-275 (2000)

Sonoyama,K.：“肽 YY 刺激 Caco-2 肠细胞中​​载脂蛋白 A-IV 基因的表达”Proc.Soc.Exp.Biol.Med.. 223. 270-275 (2000)

Sonoyama, K.: "Regulation of apolipoprotein in intestine"J.Jpn.Soc.Nutr.Food Sci.. 53. 65-70 (2000)

Sonoyama, K.：“肠内载脂蛋白的调节”J.Jpn.Soc.Nutr.Food Sci.. 53. 65-70 (2000)

Sonoyama: "Intravenous infusion of hexamethonium and atropine but not propranolol diminished apolipoprotein A-IV gene expression in rat ileum"J.Nutr.. (印刷中).

Sonoyama：“静脉输注六甲铵和阿托品而非普萘洛尔可降低大鼠回肠载脂蛋白 A-IV 基因表达”J. Nutr..（出版中）。

園山慶: "消化管におけるアポリポタンパク質の発現調節に関する研究"日本栄養・食糧学会誌. 53. 65-70 (2000)

Kei Sonoyama：“胃肠道中载脂蛋白表达调节的研究”日本营养食品学会杂志 53. 65-70 (2000)。