Mechanism of controlling blood-cell passage between the sinus endothelial cells
控制窦内皮细胞之间血细胞通道的机制
基本信息
- 批准号:11670028
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The fine structure of the tight junctions between the rat sinus endothelial cells was examined using freeze-fracture, triton extraction, and lanthanum-tracer techniques. In freeze-fracture replicas, the segmented strands and grooves of the tight junctions were frequently observed on the basolateral surfaces of the sinus endothelial cells. Some strands or grooves were discontinuous and were not closed. In the vertical sections of the sinus endothelial cells treated with lanthanum nitrate, most of them were situated on the basal part of the lateral surfaces of the adjacent endothelial cells. The intercellular spaces of the adjacent endothelial cells except for the fusion of the junctional membranes, were electron dense and the infiltration of lanthanum nitrate was found not to be interrupted by these tight junctions.The existence of the surface connected canalicular system (SCCS) in the sinus endothelial cells has been demonstrated by transmission electron microscopy using lanthanum nitr … More ate and, the three dimensional arrangement of the canaliculi was also demonstrated by computer-aided reconstruction. The SCCS of the sinus endothelial cells consisted of slender canaliculi which were branched, anastomosed, and showed continuity with the plasma membrane. From a computer-aided reconstruction the canaliculi, which were invaginated from various sites of the plasma membrane, were found to be continuous with each other and penetrate the sinus endothelial cell, to form a complex network in the cytoplasm.The immunolocalization of caveolin-3 in the sinus endothelial cells was examined with confocal laser scanning and electron microscopes. In immunofluorescence microscopy of the sinus endothelial cells, the contours of the endothelial cells were labelled with anti-caveolin-3. Caveolin-3 was present near stress fibers in the cytoplasm of the endothelial cells in a speckled pattern. Immunogold electron microscopy of ultrathin cryosections revealed anti-caveolin-3 labeling in caveolae in the apical, lateral, and basal plasma membranes and some vesicular profiles in the cytoplasm of the sinus endothelial cells. Less
采用冷冻断裂法、Triton抽提法和镧示踪技术研究了大鼠窦内皮细胞之间紧密连接的精细结构。在冷冻断裂复制品中,经常在窦内皮细胞的基底外侧表面上观察到紧密连接的分段链和凹槽。一些股线或凹槽不连续且不闭合。在硝酸镧处理的窦内皮细胞的垂直切片中,大多数位于相邻内皮细胞侧面的基底部。窦内皮细胞间除连接膜融合外,细胞间隙电子致密,硝酸镧的浸润不受这些紧密连接的影响。用硝酸镧作示踪剂,用透射电镜观察窦内皮细胞表面连接小管系统(SCCS)的存在。结果表明,在窦内皮细胞中,SCCS的存在与硝酸镧的作用有关。 ...更多信息 同时,计算机辅助重建显示了泪小管的三维结构。窦内皮细胞的SCCS由细长的小管组成,小管分支,闭合,与质膜连续。计算机辅助重建的结果显示,从质膜不同部位内陷的小管相互连续,并穿过窦内皮细胞,在细胞质中形成复杂的网络结构,用激光共聚焦扫描和电子显微镜观察了caveolin-3在窦内皮细胞中的免疫定位。在窦内皮细胞的免疫荧光显微镜下,用抗小窝蛋白-3标记内皮细胞的轮廓。Caveolin-3在内皮细胞胞质中呈斑点状分布于应力纤维附近。免疫金电子显微镜检查显示抗小窝蛋白-3标记的小窝在顶端,侧,和基底质膜和一些泡状的配置文件中的窦内皮细胞的细胞质。少
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Uehara K.and Miyoshi M.: "The surface-connected canalicular system in the sinus endothelial cells of rat spleen"Cell Tissue Res.. 296. 439-442 (1999)
Uehara K.和Miyoshi M.:“大鼠脾窦内皮细胞中的表面连接小管系统”细胞组织研究296. 439-442 (1999)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Uehara K.and Miyoshi M.: "Tight junction of sinus endothelial cells of the rat spleen"Tissue & Cell. 31. 555-560 (1999)
Uehara K.和Miyoshi M.:“大鼠脾窦内皮细胞的紧密连接”组织
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
K.Uehara & M.Miyoshi: "Tubular invaginations with caveolae and coated pirs in the sinus endothelial cells of the rat spleen"Histochem Cell Biol. 112. 351-358 (1999)
上原健
- DOI:
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- 影响因子:0
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UEHARA Kiyoko其他文献
UEHARA Kiyoko的其他文献
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{{ truncateString('UEHARA Kiyoko', 18)}}的其他基金
Analysis of dynamics of splenic sinus endothelial cells for controlling of blood cell passage
脾窦内皮细胞控制血细胞通过的动力学分析
- 批准号:
16590159 - 财政年份:2004
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Ultrastructural analysis of the mechanism of the contraction of splenic sinus endothelial cells
脾窦内皮细胞收缩机制的超微结构分析
- 批准号:
14570032 - 财政年份:2002
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of controlling blood-cell passage between the sinus endothelial cells
控制窦内皮细胞之间血细胞通道的机制
- 批准号:
09670034 - 财政年份:1997
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of controlling blood-cell passage between the sinus endothelial cells
控制窦内皮细胞之间血细胞通道的机制
- 批准号:
07807005 - 财政年份:1995
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Three Dimensional Structure of Microridges of the oral mucosal epithelium
口腔粘膜上皮微脊的三维结构
- 批准号:
03670860 - 财政年份:1991
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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