Three Dimensional Structure of Microridges of the oral mucosal epithelium

口腔粘膜上皮微脊的三维结构

• 批准号: 03670860
• 负责人: UEHARA Kiyoko
• 金额: $ 1.28万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670860/
• 关键词: Oral mucosa; Microridges; Cytoskeleton; Actin filament; Keratin filament; Microtubules; Mucous secretory granules; Intracellular transport; アクテンフィラメント; 微小菅; 粘液分泌; 密着結合; 微細構造; コイ Cyprinus carpio(硬骨魚); アクチンフイラメント; ケラチンフイラメント; コイ cyprinus car

Superficial cells of the fish oral mucosal epithelium in the carp and cytoskeleton of the epithelial cells are examined by scanning and transmission electron microscopy. Microridges are formed on the surface of the epithelium. Epithelial cells contain two types vesicles : mucous secretory vesicles and coated vesicles. Most of the mucous vesicles are situated in the center of the cell near the Golgi apparatus. In freeze-fracture replicas, intramembranous particles are abundant in the membranes of the secretory vesicles but rare in the apical plasma membrane. Coated vesicles are situated in the apical and subapical cytoplasm. A great number of thick filaments, considered to be keratin filaments, run randomly throughout the cell to form a meshwork. Thick filaments, which are sparse in the central cytoplasm, are connected to the membranes of the secretory vesicles and other membranous organelles. A layr of closely packed thin filaments, considered to be actin filaments, is found just beneath the apical plasma membrane. Microtubules also occur in the apical cytoplasm and run almost parallel to the cell surface. Both kinds of vesicles are connected to thin and thick filaments.

用扫描电镜和透射电镜观察了鲤鱼口腔粘膜上皮的表层细胞及其细胞骨架。微脊形成于上皮表面。上皮细胞含有两种囊泡：粘液分泌囊泡和被膜囊泡。大多数粘液囊泡位于细胞中央靠近高尔基体的位置。在冷冻断裂复制品，膜内颗粒丰富的分泌囊泡的膜，但在顶端质膜罕见。包被小泡位于顶端和近顶端的细胞质中。大量的粗丝（被认为是角蛋白丝）在整个细胞中随机分布，形成网状结构。在中央细胞质中稀疏的粗丝与分泌囊泡和其他膜性细胞器的膜相连。一层紧密排列的细丝，被认为是肌动蛋白丝，被发现就在顶端质膜之下。微管也出现在顶端细胞质中，几乎平行于细胞表面。这两种囊泡都连接到薄的和厚的细丝上。

项目成果

K.Uehara,M.Miyoshi,S.Miyoshi: "Function of the cytoskeleton in cells with micro-ridges from the oral epithelium of the carp Cyprinus carpio" Cell and Tissue Research. (印刷中). (1994)

K. Uehara、M. Miyoshi、S. Miyoshi：“鲤鱼口腔上皮微脊细胞骨架的功能”，细胞与组织研究（1994 年出版）。

K.Uehara, M.Miyoshi, S.Miyoshi: "Function of the cytoskeleton in cells with microridges from the oral epithelium of the carp Cyprinus carpio" Cell Tissue Res.In press.

K.Uehara、M.Miyoshi、S.Miyoshi：“鲤鱼口腔上皮微脊细胞中细胞骨架的功能”细胞组织研究出版社。

K.Uehara,M.Miyoshi,S.Miyoshi: "Function of the cytoskeletonin cells with microridges from the oral epithelium of the carp Syprimus carpio" Cell and Tissue Research. 印刷中. (1994)

K. Uehara、M. Miyoshi、S. Miyoshi：“鲤鱼口腔上皮微脊细胞骨架的功能”，细胞与组织研究，出版中。

Kiyoko Uehara et al.: "Cytoskeleton in micoridges of the oral mucosal epitelium in the carp,Cyprinus carpio" The Anatomical Record. 230. 164-168 (1991)

Kiyoko Uehara 等人：“鲤鱼口腔粘膜上皮微脊中的细胞骨架，Cyprinus carpio”解剖记录。